Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken.
Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken.
复制标题
鸡的多药耐药性大肠杆菌的毒力和转录组轮廓。
DOI：
10.1038/s41598-017-07798-1
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发表时间：
2017-08-21
影响因子：
4.6
通讯作者：
Yuan Z
中科院分区：
文献类型：
--
作者：
Hussain HI;Iqbal Z;Seleem MN;Huang D;Sattar A;Hao H;Yuan Z
许多研究已经检测了致病性大肠杆菌在家禽和家禽产品中的流行率；然而，关于它们的耐药性和毒力相关基因表达谱的数据有限。本研究旨在通过对家禽大肠杆菌Caco-2和RAW 264.7细胞株的抗生素敏感性、生物膜的形成和粘附性、侵袭力和细胞内存活率的测定，以及2日龄鸡的半数致死量的测定，来检测家禽大肠杆菌的体内外耐药性和毒力。从肉鸡中分离的临床致病多药耐药菌株E.Coli381在细胞培养中被发现具有高度的毒力，在鸡模型中的毒力是其他菌株的1000倍；因此，该菌株随后被选作转录组分析。用Illumina HiSeq完成多药耐药E.coli381与人参比菌株E.coliATCC 25922的基因表达谱比较。2500个转录组分析。差异基因表达分析表明，该高毒力菌株的抗性和毒力涉及多条途径。这项研究的结果提供了有关家禽来源的高毒力多药耐药大肠杆菌株的关键信息，由于其对公众健康构成严重威胁，因此值得进一步研究。
Numerous studies have examined the prevalence of pathogenic Escherichia coli in poultry and poultry products; however, limited data are available regarding their resistance- and virulence-associated gene expression profiles. This study was designed to examine the resistance and virulence of poultry E. coli strains in vitro and in vivo via antibiotic susceptibility, biofilm formation and adhesion, and invasion and intracellular survivability assays in Caco-2 and Raw 264.7 cell lines as well as the determination of the median lethal dose in two-day old chickens. A clinical pathogenic multidrug-resistant isolate, E. coli 381, isolated from broilers, was found to be highly virulent in cell culture and 1000-fold more virulent in a chicken model than other strains; accordingly, the isolate was subsequently selected for transcriptome analysis. The comparative gene expression profile of MDR E. coli 381 and the reference human strain E. coli ATCC 25922 was completed with Illumina HiSeq. 2500 transcriptome analysis. Differential gene expression analysis indicates that there are multiple pathways involved in the resistance and virulence of this highly virulent strain. The results garnered from this study provide critical information about the highly virulent MDR E. coli strain of poultry origin and warrant further investigation due to its significant threat to public health.
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作者：
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通讯作者：
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