The effect of RGD peptide on 2D and miniaturized 3D culture of HEPM cells, MSCs, and ADSCs with alginate hydrogel.
The effect of RGD peptide on 2D and miniaturized 3D culture of HEPM cells, MSCs, and ADSCs with alginate hydrogel.
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DOI：
10.1007/s12195-016-0428-9
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发表时间：
2016-06
影响因子：
2.8
通讯作者：
He, Xiaoming
中科院分区：
文献类型：
--
作者：
Dumbleton, Jenna;Agarwal, Pranay;Huang, Haishui;Hogrebe, Nathaniel;Han, Renzhi;Gooch, Keith J.;He, Xiaoming
组织工程的进步需要开发新技术来研究体外细胞行为。本研究重点关注藻酸盐生物材料的各种微型三维 (3D) 培养条件下的干细胞行为，这些藻酸盐生物材料经过精氨酸-甘氨酸-天冬氨酸 (RGD) 肽修饰，该肽因其在细胞粘附/附着中的作用而闻名。将人胚胎腭间充质 (HEPM) 细胞、骨髓源性间充质干细胞 (MSC) 和人脂肪源性干细胞 (ADSC) 培养在不同浓度海藻酸盐-RGD 的扁平水凝胶上，以及具有 2% 海藻酸盐或 2% 海藻酸盐-RGD 壳的微型 3D 微胶囊核心中。核心由 0%、0.5% 或 2% 海藻酸盐-RGD 制成。在所有含有 RGD 肽的系统中观察到细胞铺展，并通过测量细胞表面积和圆形度来量化细胞形态。在所有类型的干细胞中，在藻酸盐-RGD 条件下，细胞表面积显着增加（p < 0.05），细胞圆形度显着降低（p < 0.01），表明细胞在含有该肽的环境中更容易扩散。这种对 3D 微环境中细胞扩散的控制有助于创造理想的仿生条件，以便对细胞行为进行进一步研究。
Advancements in tissue engineering require the development of new technologies to study cell behavior in vitro. This study focuses on stem cell behavior within various miniaturized three-dimensional (3D) culture conditions of alginate biomaterials modified with the Arg-Gly-Asp (RGD) peptide known for its role in cell adhesion/attachment. Human embryonic palatal mesenchyme (HEPM) cells, bone marrow derived mesenchymal stem cells (MSCs), and human adipose derived stem cells (ADSCs) were cultured on a flat hydrogel of different concentrations of alginate-RGD, and in the miniaturized 3D core of microcapsules with either a 2% alginate or 2% alginate-RGD shell. The core was made of 0%, 0.5%, or 2% alginate-RGD. Cell spreading was observed in all systems containing the RGD peptide, and the cell morphology was quantified by measuring the cell surface area and circularity. In all types of stem cells, there was a significant increase in the cell surface area (p < 0.05) and a significant decrease in cell circularity (p < 0.01) in alginate-RGD conditions, indicating that cells spread much more readily in environments containing the peptide. This control over the cell spreading within a 3D microenvironment can help to create the ideal biomimetic condition in which to conduct further studies on cell behavior.
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影响因子：
4
作者：
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