Signature of circulating microRNAs as potential biomarkers in vulnerable coronary artery disease.
Signature of circulating microRNAs as potential biomarkers in vulnerable coronary artery disease.
复制标题
循环 MicroRNA 的特征作为脆弱冠状动脉疾病的潜在生物标志物
DOI：
10.1371/journal.pone.0080738
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Ren J;Zhang J;Xu N;Han G;Geng Q;Song J;Li S;Zhao J;Chen H
AIMS microRNAs(MiRNAs)在心血管疾病的发病机制中发挥重要作用。近年来，循环中的miRNAs被认为是各种生理和病理状态的生物标志物。在这项研究中，我们旨在鉴定易损冠状动脉疾病(CAD)的循环miRNA指纹，并探索其作为该疾病新的生物标志物的潜力。方法和结果应用Taqman低密度miRNA阵列和共表达网络分析方法鉴定典型不稳定型心绞痛患者(UA组，n = 13)和非心源性胸痛患者(对照组，n = 13)血浆中miRNA的表达谱。UA组miR-106b/25簇、miR-17/92a簇、miR-21/590-5p家族、miR-126*和miR-451的表达水平显著高于对照组。这些发现在另外45名不稳定型心绞痛患者、31名稳定型心绞痛患者和37名对照组中得到了实时聚合酶链式反应的验证。此外，UA患者(n = 5)分离的微粒子(MPS)中miR-106b、miR-25、miR-92a、miR-21、miR-5p、miR-126*和miR-451的表达均较对照组(n = 5)上调。应用流式细胞仪和免疫标记进一步发现UA患者血浆中Annexin V+MPS明显高于对照组，且以Annexin V+CD31+MPS为主。提示Annexin V+CD31+MPS可能是易损性冠心病患者循环中部分miRNAs表达升高的原因之一。结论由miR-106b/25簇、miR-17/92a簇、miR-21/590-5p家族、miR-126*和miR-451组成的循环miRNA信号可作为冠心病易损性的一种新的生物标志物。中国临床试验注册登记，CHICCTR-OCH-12002349。
Aims MicroRNAs (miRNAs) play important roles in the pathogenesis of cardiovascular diseases. Circulating miRNAs were recently identified as biomarkers for various physiological and pathological conditions. In this study, we aimed to identify the circulating miRNA fingerprint of vulnerable coronary artery disease (CAD) and explore its potential as a novel biomarker for this disease. Methods and Results The Taqman low-density miRNA array and coexpression network analyses were used to identify distinct miRNA expression profiles in the plasma of patients with typical unstable angina (UA) and angiographically documented CAD (UA group, n = 13) compared to individuals with non-cardiac chest pain (control group, n = 13). Significantly elevated expression levels of miR-106b/25 cluster, miR-17/92a cluster, miR-21/590-5p family, miR-126*, and miR-451 were observed in UA patients compared to controls. These findings were validated by real-time PCR in another 45 UA patients, 31 stable angina patients, and 37 controls. In addition, miR-106b, miR-25, miR-92a, miR-21, miR-590-5p, miR-126* and miR-451 were upregulated in microparticles (MPs) isolated from the plasma of UA patients (n = 5) compared to controls (n = 5). Using flow cytometry and immunolabeling, we further found that Annexin V+ MPs were increased in the plasma samples of UA patients compared to controls, and the majority of the increased MPs in plasma were shown to be Annexin V+ CD31+ MPs. The findings suggest that Annexin V+ CD31+ MPs may contribute to the elevated expression of the selected miRNAs in the circulation of patients with vulnerable CAD. Conclusion The circulating miRNA signature, consisting of the miR-106b/25 cluster, miR-17/92a cluster, miR-21/590-5p family, miR-126* and miR-451, may be used as a novel biomarker for vulnerable CAD. Trial Registration Chinese Clinical Trial Register, ChiCTR-OCH-12002349.
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影响因子：
14.9
作者：
Kozomara A;Griffiths-Jones S
通讯作者：
Griffiths-Jones S
DOI：
10.1161/atvbaha.111.226696
发表时间：
2011-11-01
影响因子：
8.7
作者：
Fichtlscherer, Stephan;Zeiher, Andreas M.;Dimmeler, Stefanie
通讯作者：
Dimmeler, Stefanie
早发冠心病患者的血小板表现出 miRNA340* 和 miRNA624* 的上调。
影响因子：
3.7
作者：
Sondermeijer BM;Bakker A;Halliani A;de Ronde MW;Marquart AA;Tijsen AJ;Mulders TA;Kok MG;Battjes S;Maiwald S;Sivapalaratnam S;Trip MD;Moerland PD;Meijers JC;Creemers EE;Pinto-Sietsma SJ
通讯作者：
Pinto-Sietsma SJ
影响因子：
3.9
作者：
Rippe, Catarina;Blimline, Mark;Magerko, Katherine A.;Lawson, Brooke R.;LaRocca, Thomas J.;Donato, Anthony J.;Seals, Douglas R.
通讯作者：
Seals, Douglas R.
影响因子：
11.5
作者：
Bandres, Eva;Bitarte, Nerea;Garcia-Foncillas, Jesus
通讯作者：
Garcia-Foncillas, Jesus