Platelets in patients with premature coronary artery disease exhibit upregulation of miRNA340* and miRNA624*.
Platelets in patients with premature coronary artery disease exhibit upregulation of miRNA340* and miRNA624*.
复制标题
早发冠心病患者的血小板表现出 miRNA340* 和 miRNA624* 的上调。
DOI：
10.1371/journal.pone.0025946
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Pinto-Sietsma SJ
中科院分区：
文献类型：
--
作者：
Sondermeijer BM;Bakker A;Halliani A;de Ronde MW;Marquart AA;Tijsen AJ;Mulders TA;Kok MG;Battjes S;Maiwald S;Sivapalaratnam S;Trip MD;Moerland PD;Meijers JC;Creemers EE;Pinto-Sietsma SJ
冠状动脉疾病（CAD）是全世界人类发病和死亡的主要原因，这凸显了改进诊断策略的必要性。血小板不仅在斑块破裂期间的急性血栓形成过程中发挥着重要作用，而且在动脉粥样硬化本身的形成中也发挥着重要作用。 MicroRNA 是控制基因表达并以组织和疾病特异性方式表达的内源性小非编码 RNA。因此，它们被认为是有用的生物标志物。目前尚不清楚早发 CAD 患者和健康对照者之间是否存在血小板中 miRNA 表达水平的差异。在这项病例对照研究中，我们使用 12 名早发 CAD 患者和 12 名年龄和性别匹配的健康对照的微阵列测量了血小板 miRNA 的相对表达水平。与健康对照相比，CAD 患者中 6 种血小板 microRNA 显着上调（miR340*、miR451、miR454*、miR545:9.1、miR615-5p 和 miR624*），一种 miRNA (miR1280) 显着下调。为了验证这些结果，我们通过 qRT-PCR 测量了来自两个独立队列的个体血小板中这些候选 miRNA 的表达水平；验证队列 I 由 40 名早发 CAD 患者和 40 名健康对照组成，验证队列 II 包括 27 名动脉疾病患者和 40 名健康亲属。在两个验证队列中，与健康对照相比，miR340* 和 miR624* 被证实在 CAD 患者中表达上调。与健康对照相比，CAD 患者血小板中的两种 miRNA 显着上调。这两种鉴定出的 miRNA 是否可以用作生物标志物，以及它们是疾病的原因还是结果，仍有待在更大规模的前瞻性研究中阐明。
Coronary artery disease (CAD) is the leading cause of human morbidity and mortality worldwide, underscoring the need to improve diagnostic strategies. Platelets play a major role, not only in the process of acute thrombosis during plaque rupture, but also in the formation of atherosclerosis itself. MicroRNAs are endogenous small non-coding RNAs that control gene expression and are expressed in a tissue and disease-specific manner. Therefore they have been proposed to be useful biomarkers. It remains unknown whether differences in miRNA expression levels in platelets can be found between patients with premature CAD and healthy controls. In this case-control study we measured relative expression levels of platelet miRNAs using microarrays from 12 patients with premature CAD and 12 age- and sex-matched healthy controls. Six platelet microRNAs were significantly upregulated (miR340*, miR451, miR454*, miR545:9.1. miR615-5p and miR624*) and one miRNA (miR1280) was significantly downregulated in patients with CAD as compared to healthy controls. To validate these results, we measured the expression levels of these candidate miRNAs by qRT-PCR in platelets of individuals from two independent cohorts; validation cohort I consisted of 40 patients with premature CAD and 40 healthy controls and validation cohort II consisted of 27 patients with artery disease and 40 healthy relatives. MiR340* and miR624* were confirmed to be upregulated in patients with CAD as compared to healthy controls in both validation cohorts. Two miRNAs in platelets are significantly upregulated in patients with CAD as compared to healthy controls. Whether the two identified miRNAs can be used as biomarkers and whether they are cause or consequence of the disease remains to be elucidated in a larger prospective study.
登录
查看更多内容
影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
DOI：
10.3324/haematol.12706
发表时间：
2008-07-01
期刊：
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL
影响因子：
--
作者：
Bruchova, Hana;Merkerova, Michaela;Prchal, Josef T.
通讯作者：
Prchal, Josef T.
影响因子：
9.3
作者：
Adachi, Taichi;Nakanishi, Michio;Iwai, Naoharu
通讯作者：
Iwai, Naoharu
影响因子：
30.8
作者：
Bentwich, I;Avniel, A;Bentwich, Z
通讯作者：
Bentwich, Z
影响因子：
--
作者：
Corsten, Maarten F.;Dennert, Robert;Schroen, Blanche
通讯作者：
Schroen, Blanche