Spatial analysis of slowly oscillating electric activity in the gut of mice using low impedance arrayed microelectrodes.
Spatial analysis of slowly oscillating electric activity in the gut of mice using low impedance arrayed microelectrodes.
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DOI：
10.1371/journal.pone.0075235
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nakayama S
中科院分区：
文献类型：
--
作者：
Taniguchi M;Kajioka S;Shozib HB;Sawamura K;Nakayama S
顺畅和精细的肠道运动是基于细胞的合作，包括平滑肌，肠神经元和特殊的间质细胞作为起搏细胞。因此，空间表征的电活动的组织中含有这些电兴奋细胞是需要一个精确的理解肠道运动。此外，在一个小区域内评估空间电活动的工具将有助于模型动物的研究。因此，我们采用了微电极阵列（MEA）系统，同时测量一组8×8场电位在一个平方米的平方毫米。每个记录电极的尺寸为50×50 µm2，但是通过固定铂黑颗粒增加了表面积。微电极的阻抗足够低以应用0.1Hz的高通滤波器。光谱功率映射以及自相关和互相关参数表征了野生型（WT）和W/Wv小鼠回肠自发电活动的空间特性，后者用作起搏间质细胞网络受损的模型。也就是说，在W/Wv小鼠中测量的电活动在大小和协同性上都不同，尽管面积很小。在野生型小鼠的回肠中，抑制平滑肌和神经元兴奋性的程序改变了自发电活动的传播，但在振荡期间几乎没有变化。总之，具有低阻抗电极的MEA能够测量缓慢振荡的电活动，并且对于评估肠电活动的时空特性的组织学和功能变化是有用的。
Smooth and elaborate gut motility is based on cellular cooperation, including smooth muscle, enteric neurons and special interstitial cells acting as pacemaker cells. Therefore, spatial characterization of electric activity in tissues containing these electric excitable cells is required for a precise understanding of gut motility. Furthermore, tools to evaluate spatial electric activity in a small area would be useful for the investigation of model animals. We thus employed a microelectrode array (MEA) system to simultaneously measure a set of 8×8 field potentials in a square area of ∼1 mm2. The size of each recording electrode was 50×50 µm2, however the surface area was increased by fixing platinum black particles. The impedance of microelectrode was sufficiently low to apply a high-pass filter of 0.1 Hz. Mapping of spectral power, and auto-correlation and cross-correlation parameters characterized the spatial properties of spontaneous electric activity in the ileum of wild-type (WT) and W/Wv mice, the latter serving as a model of impaired network of pacemaking interstitial cells. Namely, electric activities measured varied in both size and cooperativity in W/Wv mice, despite the small area. In the ileum of WT mice, procedures suppressing the excitability of smooth muscle and neurons altered the propagation of spontaneous electric activity, but had little change in the period of oscillations. In conclusion, MEA with low impedance electrodes enables to measure slowly oscillating electric activity, and is useful to evaluate both histological and functional changes in the spatio-temporal property of gut electric activity.
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