Identification and Characterization of Neuropeptides by Transcriptome and Proteome Analyses in a Bivalve Mollusc Patinopecten yessoensis.
Identification and Characterization of Neuropeptides by Transcriptome and Proteome Analyses in a Bivalve Mollusc Patinopecten yessoensis.
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通过转录组和蛋白质组分析对双壳类软体动物虾夷扇贝神经肽进行鉴定和表征
DOI：
10.3389/fgene.2018.00197
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发表时间：
2018
影响因子：
3.7
通讯作者：
Bao Z
中科院分区：
文献类型：
--
作者：
Zhang M;Wang Y;Li Y;Li W;Li R;Xie X;Wang S;Hu X;Zhang L;Bao Z
Neuropeptides play essential roles in regulation of reproduction and growth in marine molluscs. But their function in marine bivalves – a group of animals of commercial importance – is largely unexplored due to the lack of systematic identification of these molecules. In this study, we sequenced and analyzed the transcriptome of nerve ganglia of Yesso scallop Patinopecten yessoensis, from which 63 neuropeptide genes were identified based on BLAST and de novo prediction approaches, and 31 were confirmed by proteomic analysis using the liquid chromatography-tandem mass spectrometry (LC-MS/MS). Fifty genes encode known neuropeptide precursors, of which 20 commonly exist in bilaterians and 30 are protostome specific. Three neuropeptides that have not yet been reported in bivalves were identified, including calcitonin/DH31, lymnokinin and pleurin. Characterization of glycoprotein hormones, insulin-like peptides, allatostatins, RFamides, and some reproduction, cardioactivity or feeding related neuropeptides reveals scallop neuropeptides have conserved molluscan neuropeptide domains, but some (e.g., GPB5, APGWamide and ELH) are characterized with bivalve-specific features. Thirteen potentially novel neuropeptides were identified, including 10 that may also exist in other protostomes, and 3 (GNamide, LRYamide, and Vamide) that may be scallop specific. In addition, we found neuropeptides potentially related to scallop shell growth and eye functioning. This study represents the first comprehensive identification of neuropeptides in scallop, and would contribute to a complete understanding on the roles of various neuropeptides in endocrine regulation in bivalve molluscs.
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影响因子：
5.2
作者：
Elphick MR;Mirabeau O
通讯作者：
Mirabeau O
影响因子：
4.8
作者：
De Jong-Brink, M;Reid, CN;Ter Maat, A
通讯作者：
Ter Maat, A
影响因子：
1.6
作者：
CANDELARIOMARTINEZ, A;REED, DM;GREENBERG, MJ
通讯作者：
GREENBERG, MJ
影响因子：
3
作者：
Di Cristo, C;Delli Bovi, P;Di Cosmo, A
通讯作者：
Di Cosmo, A
影响因子：
4.4
作者：
Conzelmann M;Williams EA;Krug K;Franz-Wachtel M;Macek B;Jékely G
通讯作者：
Jékely G