Development of an Electrochemical Paper-Based Analytical Device for Trace Detection of Virus Particles.
Development of an Electrochemical Paper-Based Analytical Device for Trace Detection of Virus Particles.
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DOI：
10.1021/acs.analchem.8b02042
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发表时间：
2018-06-19
影响因子：
7.4
通讯作者：
Henry CS
中科院分区：
文献类型：
--
作者：
Channon RB;Yang Y;Feibelman KM;Geiss BJ;Dandy DS;Henry CS
病毒性病原体在世界各地都是严重的健康威胁，特别是在资源有限的环境中，目前的传感方法往往不足和缓慢，加剧了这些病原体的传播和负担。在这里，我们描述了一种基于集成微线金电极的微流控纸分析装置的无标签、即时检测病毒颗粒的方法。该装置最初的特点是通过生物素修饰的微丝捕获链霉亲和素修饰的纳米颗粒。由于改进的电极上的质量传递和颗粒捕获增强，通过使用微流控装置，检测限的数量级提高了，而不是传统的静态纸基装置。采用抗体功能化Au微线对西尼罗病毒颗粒进行电化学阻抗谱检测，在50 μL细胞培养基中，检测限为10.2个颗粒。在添加过量的非特异性靶标（Sindbis）时，没有发现信号的增加。这种检测基序比目前的方法更便宜（每次测试约1美元），更快（约30分钟），同时达到所需的选择性和灵敏度。这种传感基序代表了广泛的生物病原体痕量检测的通用平台。
Viral pathogens are a serious health threat around the world, particularly in resource limited settings, where current sensing approaches are often insufficient and slow, compounding the spread and burden of these pathogens. Here, we describe a label-free, point-of-care approach toward detection of virus particles, based on a microfluidic paper-based analytical device with integrated microwire Au electrodes. The device is initially characterized through capturing of streptavidin modified nanoparticles by biotin-modified microwires. An order of magnitude improvement in detection limits is achieved through use of a microfluidic device over a classical static paper-based device, due to enhanced mass transport and capturing of particles on the modified electrodes. Electrochemical impedance spectroscopy detection or West Nile virus particles was carried out using antibody runctionalized Au microwires, achieving a detection limit of 10.2 particles in 50 μL of cell culture media. No increase in signal is found on addition of an excess of a nonspecific target (Sindbis). This detection motif is significantly cheaper (~$1 per test) and faster (~30 min) than current methods, while achieving the desired selectivity and sensitivity. This sensing motif represents a general platform for trace detection of a wide range of biological pathogens.
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DOI：
10.3390/s17112597
发表时间：
2017-11-11
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Devarakonda S;Singh R;Bhardwaj J;Jang J
通讯作者：
Jang J
影响因子：
8.4
作者：
Jiang, Jing;Wang, Xinhao;Liu, Gang Logan
通讯作者：
Liu, Gang Logan
影响因子：
6.4
作者：
Barzon, Luisa;Pacenti, Monia;Palu, Giorgio
通讯作者：
Palu, Giorgio
影响因子：
8.9
作者：
Kelley, Shana O.
通讯作者：
Kelley, Shana O.
影响因子：
12.6
作者：
Gupta, Rakesh K.;Periyakaruppan, Adaikkappan;Meyyappan, M.;Koehne, Jessica E.
通讯作者：
Koehne, Jessica E.