Cost-Effective and Handmade Paper-Based Immunosensing Device for Electrochemical Detection of Influenza Virus.
Cost-Effective and Handmade Paper-Based Immunosensing Device for Electrochemical Detection of Influenza Virus.
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DOI：
10.3390/s17112597
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发表时间：
2017-11-11
期刊：
影响因子：
--
通讯作者：
Jang J
中科院分区：
文献类型：
--
作者：
Devarakonda S;Singh R;Bhardwaj J;Jang J
尽管许多关于流感病毒检测的研究已经发表，但基于纸张的无标记电化学免疫传感器尚未见报道。在此，我们介绍一种用于流感病毒H1N1无标记电化学检测的具有成本效益的手工制作的纸质免疫传感器。这种免疫传感器是通过用疏水性二氧化硅纳米粒子喷雾修饰纸张，并使用模板印刷电极制备的。我们使用玻璃蒸发器将疏水性二氧化硅纳米粒子喷到纸张上，使其具有超疏水性。这种对于纸质生物传感器至关重要的超疏水性是通过30 - 40次喷涂实现的，这相当于在纸张上有0.39 - 0.41毫克/平方厘米的纳米粒子涂层，并且产生150°±1°的水接触角。采用单壁碳纳米管和壳聚糖修饰的模板印刷碳电极来提高传感器的灵敏度，并且通过戊二醛交联固定抗体。采用差分脉冲伏安法评估传感器在10到10⁴ PFU/毫升不同病毒浓度下的灵敏度，并针对MS2噬菌体和乙型流感病毒评估其选择性。这些免疫传感器表现出良好的线性行为、更短的检测时间（30分钟）以及对H1N1病毒的选择性，检测限为113 PFU/毫升，这对于快速现场诊断足够灵敏。本研究中开发的简单且廉价的方法具有很大潜力，可用于开发一种低成本且一次性的用于检测病原微生物的免疫传感器，特别是在发展中国家。
Although many studies concerning the detection of influenza virus have been published, a paper-based, label-free electrochemical immunosensor has never been reported. Here, we present a cost-effective, handmade paper-based immunosensor for label-free electrochemical detection of influenza virus H1N1. This immunosensor was prepared by modifying paper with a spray of hydrophobic silica nanoparticles, and using stencil-printed electrodes. We used a glass vaporizer to spray the hydrophobic silica nanoparticles onto the paper, rendering it super-hydrophobic. The super-hydrophobicity, which is essential for this paper-based biosensor, was achieved via 30–40 spray coatings, corresponding to a 0.39–0.41 mg cm−2 coating of nanoparticles on the paper and yielding a water contact angle of 150° ± 1°. Stencil-printed carbon electrodes modified with single-walled carbon nanotubes and chitosan were employed to increase the sensitivity of the sensor, and the antibodies were immobilized via glutaraldehyde cross-linking. Differential pulse voltammetry was used to assess the sensitivity of the sensors at various virus concentrations, ranging from 10 to 104 PFU mL−1, and the selectivity was assessed against MS2 bacteriophages and the influenza B viruses. These immunosensors showed good linear behaviors, improved detection times (30 min), and selectivity for the H1N1 virus with a limit of detection of 113 PFU mL−1, which is sufficiently sensitive for rapid on-site diagnosis. The simple and inexpensive methodologies developed in this study have great potential to be used for the development of a low-cost and disposable immunosensor for detection of pathogenic microorganisms, especially in developing countries.
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影响因子：
12.6
作者：
Lee D;Chander Y;Goyal SM;Cui T
通讯作者：
Cui T
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作者：
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12.6
作者：
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通讯作者：
Rozlosnik, Noemi