Inhibition of New Delhi Metallo-β-Lactamase 1 (NDM-1) Producing Escherichia coli IR-6 by Selected Plant Extracts and Their Synergistic Actions with Antibiotics.
Inhibition of New Delhi Metallo-β-Lactamase 1 (NDM-1) Producing Escherichia coli IR-6 by Selected Plant Extracts and Their Synergistic Actions with Antibiotics.
复制标题
通过选定的植物提取物及其对抗生素的协同作用，抑制新德里金属β-内酰胺酶1（NDM-1），产生大肠杆菌IR-6。
DOI：
10.3389/fmicb.2017.01580
复制
发表时间：
2017
影响因子：
5.2
通讯作者：
Parani M
中科院分区：
文献类型：
--
作者：
Chandar B;Poovitha S;Ilango K;MohanKumar R;Parani M
抗生素的不当使用已导致病原微生物耐药性的发展受到极大关注。新德里产金属β-内酰胺酶1（NDM-1）的细菌对大多数β-内酰胺抗生素具有耐药性，到目前为止，还没有新的化合物对这些细菌进行临床试验。本研究对240种药用植物叶的乙醇提取物进行了抗NDM-1大肠杆菌的活性筛选。然后测试显示抗菌活性的提取物的最小抑菌浓度（MIC）和抑菌圈。白风柴的提取物。唐，木槿（Hibiscus acetosella Welw.来自Hiern，Hibiscus cannabinus L.，芙蓉，石榴，和罗望子（Tamarindus indica L.）杀菌活性在5 ~ 15 mg/ml之间，MIC在2.56 ~ 5.12mg/ml之间。所有六种植物提取物在体外抑制NDM-1酶的活性，IC 50值在0.50和1.2 ng/μl之间。用扫描电子显微镜可以清楚地看到植物提取物对细菌细胞壁完整性的破坏。通过荧光激活细胞分选研究，膜渗透性的增加导致79.4-89.7%的细菌细胞死亡。所有植物提取物与粘菌素[抑菌分数（MIC）= 0.125-0.375]、美罗培南（MIC = 0.09-0.313）和四环素（MIC = 0.125-0.313）联用时均表现出协同作用。因此，该植物提取物可用于活性成分的鉴定，这些活性成分可作为新的抗菌化合物用于开发针对NDM-1 E的药物。大肠杆菌中，以及它们在联合治疗中的用途。
Improper use of antibiotics has led to a great concern in the development of pathogenic microbial resistance. New Delhi metallo-β-lactamase 1 (NDM-1) producing bacteria are resistant to most of the β-lactam antibiotics, and so far, no new compounds have been clinically tested against these bacteria. In this study, ethanol extracts from the leaves of 240 medicinal plant species were screened for antibacterial activity against an NDM-1 Escherichia coli strain. The extracts that showed antibacterial activity were then tested for minimum inhibitory concentrations (MICs) and zones of inhibition. The extract from Combretum albidum G. Don, Hibiscus acetosella Welw. ex Hiern, Hibiscus cannabinus L., Hibiscus furcatus Willd., Punica granatum L., and Tamarindus indica L. showed bactericidal activity between 5 and 15 mg/ml and the MIC was between 2.56 and 5.12 mg/ml. All six plant extracts inhibited activity of the NDM-1 enzyme in vitro, and the IC50 value ranged between 0.50 and 1.2 ng/μl. Disruption of bacterial cell wall integrity by the plant extracts was clearly visible with scanning electron microscopy. Increases in membrane permeability caused 79.4–89.7% bacterial cell deaths as investigated by fluorescence-activated cell sorting. All the plant extracts showed synergistic effects when combined with colistin [fractional inhibitory concentration (ΣFIC) = 0.125–0.375], meropenem (ΣFIC = 0.09–0.313), and tetracycline (ΣFIC = 0.125–0.313). Thus, the plant extracts can be fractionated for the identification of active compounds, which could be used as new antibacterial compounds for the development of drugs against NDM-1 E. coli in addition to their use in combination therapy.
登录
查看更多内容
选定的可食用植物提取物针对多药抗革兰氏阴性细菌的抗菌活性。
影响因子：
--
作者：
Djeussi DE;Noumedem JA;Seukep JA;Fankam AG;Voukeng IK;Tankeo SB;Nkuete AH;Kuete V
通讯作者：
Kuete V
影响因子：
4.9
作者：
da Silva, Cecilia Rocha;de Andrade Neto, Joao Batista;Nobre Junior, Helio Vitoriano
通讯作者：
Nobre Junior, Helio Vitoriano
影响因子：
4.3
作者：
Dey, Diganta;Debnath, Sukalyani;Hazra, Banasri
通讯作者：
Hazra, Banasri
影响因子：
4.9
作者：
Hornsey, Michael;Phee, Lynette;Wareham, David W.
通讯作者：
Wareham, David W.
DOI：
10.1016/j.ijfoodmicro.2010.04.025
发表时间：
2010-06-30
影响因子：
5.4
作者：
Kao, Tseng-Ting;Tu, Hung-Chi;Fu, Tzu-Fun
通讯作者：
Fu, Tzu-Fun