Commercialized kits to assess T-cell responses against SARS-CoV-2 S peptides. A pilot study in health care workers.
Commercialized kits to assess T-cell responses against SARS-CoV-2 S peptides. A pilot study in health care workers.
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DOI：
10.1016/j.medcli.2021.09.013
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发表时间：
2022-08-12
期刊：
影响因子：
3.9
通讯作者：
Hernandéz-González M
中科院分区：
文献类型：
--
作者：
Martínez-Gallo M;Esperalba J;Pujol-Borrell R;Sandá V;Arrese-Muñoz I;Fernández-Naval C;Antón A;Cardona V;Labrador-Horrillo M;Pumarola T;Hernandéz-González M
评估自然感染或 SARS-CoV-2 疫苗产生的保护水平至关重要，主要是针对职业接触者和弱势群体。测量 T 细胞反应可以补充目前用作保护相关性的抗体测试。我们的目的是评估经过验证的 T 细胞反应检测的可行性。其中包括 20 名卫生保健工作者 (HCW)。对 SARS-CoV-2 N 和 S 蛋白的抗体测试与市售的 S 肽全血干扰素γ释放测定 (IGRA) 并行，并确定了两种检测方法：CLIA 和 ELISA。 IGRA 测试检测到自然暴露和接种疫苗的医护人员在第一次接种疫苗后的 T 细胞反应。两种检测方法的相关性非常高（R > 0.8），灵敏度和特异性分别在 100-86% 和 100-73% 之间。尽管特异性抗体水平与 IGRA 检测在检测 SARS-CoV-2 免疫反应的能力方面具有非常高的一致性，但定量相关性相对较低。在自然感染引发的小组中，一剂疫苗足以达到免疫反应平台。其中一名 IGRA 呈阳性，而 Ig(S) 抗体阴性且接种了免疫抑制疫苗的医护人员说明了 IGRA 测试的另一个优势。全血 IGRA 测试适合自动化，是测量 SARS-CoV-2 免疫反应状态的一种有前途的附加工具；它们适用于大量样本，并可能成为保护 COVID-19 的有价值的相关因素，特别是对于面临再次感染风险的弱势群体（如医护人员）。
It is crucial to assess the levels of protection generated by natural infection or SARS-CoV-2 vaccines, mainly in individuals professionally exposed and in vulnerable groups. Measuring T-cell responses may complement antibody tests currently in use as correlates of protection. Our aim was to assess the feasibility of a validated assay of T-cell responses. Twenty health-care-workers (HCW) were included. Antibody test to SARS-CoV-2 N and S-proteins in parallel with a commercially available whole-blood-interferon-gamma-release-assay (IGRA) to S-peptides and two detection methods, CLIA and ELISA were determined. IGRA test detected T-cell responses in naturally exposed and vaccinated HCW already after first vaccination dose. The correlation by the two detection methods was very high (R > 0.8) and sensitivity and specificity ranged between 100 and 86% and 100-73% respectively. Even though there was a very high concordance between specific antibody levels and the IGRA assay in the ability to detect immune response to SARS-CoV-2, there was a relatively low quantitative correlation. In the small group primed by natural infection, one vaccine dose was sufficient to reach immune response plateau. IGRA was positive in one, with Ig(S) antibody negative vaccinated immunosuppressed HCW illustrating another advantage of the IGRA-test. Whole-blood-IGRA-tests amenable to automation and constitutes a promising additional tool for measuring the state of the immune response to SARS-CoV-2; they are applicable to large number of samples and may become a valuable correlate of protection to COVID-19, particularly for vulnerable groups at risk of being re-exposed to infection, as are health-care-workers.
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影响因子：
3.4
作者：
Garcia-Prat, Marina;Alvarez-Sierra, Daniel;Martinez-Gallo, Monica
通讯作者：
Martinez-Gallo, Monica
DOI：
10.1056/nejmoa2034545
发表时间：
2021-02-11
期刊：
The New England journal of medicine
影响因子：
--
作者：
Lumley SF;O'Donnell D;Stoesser NE;Matthews PC;Howarth A;Hatch SB;Marsden BD;Cox S;James T;Warren F;Peck LJ;Ritter TG;de Toledo Z;Warren L;Axten D;Cornall RJ;Jones EY;Stuart DI;Screaton G;Ebner D;Hoosdally S;Chand M;Crook DW;O'Donnell AM;Conlon CP;Pouwels KB;Walker AS;Peto TEA;Hopkins S;Walker TM;Jeffery K;Eyre DW;Oxford University Hospitals Staff Testing Group
通讯作者：
Oxford University Hospitals Staff Testing Group
DOI：
10.1016/j.cmi.2020.09.051
发表时间：
2021-03
期刊：
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
影响因子：
--
作者：
Petrone L;Petruccioli E;Vanini V;Cuzzi G;Najafi Fard S;Alonzi T;Castilletti C;Palmieri F;Gualano G;Vittozzi P;Nicastri E;Lepore L;Antinori A;Vergori A;Caccamo N;Cantini F;Girardi E;Ippolito G;Grifoni A;Goletti D
通讯作者：
Goletti D
影响因子：
64.5
作者：
Moderbacher, Carolyn Rydyznski;Ramirez, Sydney, I;Crotty, Shane
通讯作者：
Crotty, Shane
影响因子：
8.8
作者：
Lozano-Ojalvo D;Camara C;Lopez-Granados E;Nozal P;Del Pino-Molina L;Bravo-Gallego LY;Paz-Artal E;Pion M;Correa-Rocha R;Ortiz A;Lopez-Hoyos M;Iribarren ME;Portoles J;Rojo-Portoles MP;Ojeda G;Cervera I;Gonzalez-Perez M;Bodega-Mayor I;Montes-Casado M;Portoles P;Perez-Olmeda M;Oteo J;Sanchez-Tarjuelo R;Pothula V;Schwarz M;Brahmachary M;Tan AT;Le Bert N;Berin C;Bertoletti A;Guccione E;Ochando J
通讯作者：
Ochando J