Time series analysis of the transcriptional responses of Biomphalaria glabrata throughout the course of intramolluscan development of Schistosoma mansoni and Echinostoma paraensei.
Time series analysis of the transcriptional responses of Biomphalaria glabrata throughout the course of intramolluscan development of Schistosoma mansoni and Echinostoma paraensei.
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DOI：
10.1016/j.ijpara.2009.12.005
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发表时间：
2010-06
影响因子：
4
通讯作者：
Loker ES
中科院分区：
文献类型：
--
作者：
Hanington PC;Lun CM;Adema CM;Loker ES
一个兼容的蜗牛宿主的成功殖民地的二代吸虫毛蚴启动一个复杂的，增殖的发展计划，需要数周达到高潮的形式产生的尾蚴，一旦开始，可能会持续的剩余寿命的感染蜗牛。这种增殖性和侵入性的寄生虫是如何绕过宿主防御并建立慢性感染的？使用设计用于监测淡水蜗牛Biomphalaria glabrata的内部防御和应激相关反应的微阵列，我们进行了一项时间过程研究，以监测蜗牛暴露于两种不同吸虫物种后的反应，蜗牛对这两种吸虫物种敏感：医学上重要的曼氏血吸虫（Schistosomamansoni），例如在其幼虫发育中的孢子囊产生，或棘口吸虫（Echinostomparaensei），代表在其幼虫发育中强调营养生产。我们在8个时间点（感染后0.5、1、2、4、8、16和32天）取样。涵盖了尾蚴产生所需的时间。暴露于S. mansoni，在感染后2天，上调的阵列特征相对于下调的阵列特征具有优势。但在p.i.此后，这一模式被强烈逆转。大肠在paraensei中，即使在感染后0.5天，下调的阵列特征也比上调的特征占优势，除了感染后1天外，在整个感染过程中持续存在的模式，当上调的阵列特征的数量略多于下调的特征时。特定阵列特征的检查揭示了在感染过程早期被两种寄生虫上调的几种，以及在任一寄生虫感染过程中保持显著升高的纤维蛋白原相关蛋白4（FREP 4），其有效地用作感染的标志物。许多防御相关的转录持续下调，包括几个含纤维蛋白原的凝集素和同源分子最有名的脊椎动物吞噬细胞。我们的结果与早期的研究一致，表明这两种寄生虫都能够干扰宿主的防御反应，包括E。paraensei这样做比S更迅速和强烈。mansoni他们进一步提出了吸虫如何能够建立慢性感染以持续成功的机制。
Successful colonization of a compatible snail host by a digenetic trematode miracidium initiates a complex, proliferative development program requiring weeks to reach culmination in the form of production of cercariae which, once started, may persist for the remainder of the life span of the infected snail. How are such proliferative and invasive parasites able to circumvent host defenses and establish chronic infections? Using a microarray designed to monitor the internal defense and stress-related responses of the freshwater snail Biomphalaria glabrata, we have undertaken a time course study to monitor snail responses following exposure to two different trematode species to which the snail is susceptible: the medically important Schistosoma mansoni, exemplifying sporocyst production in its larval development, or Echinostoma paraensei, representing an emphasis on rediae production in its larval development. We sampled eight time points (0.5, 1, 2, 4, 8, 16 and 32 days p.i.) that cover the period required for cercariae to be produced. Following exposure to S. mansoni, there was a preponderance of up-regulated over down-regulated array features through 2 days p.i. but by 4 days p.i. and thereafter, this pattern was strongly reversed. For E. paraensei, there was a preponderance of down-regulated array features over up-regulated features at even 0.5 days p.i., a pattern that persists throughout the course of infection except for 1 day p.i., when up-regulated array features slightly outnumbered down-regulated features. Examination of particular array features revealed several that were up-regulated by both parasites early in the course of infection and one, fibrinogen related protein 4 (FREP 4), that remained significantly elevated throughout the course of infection with either parasite, effectively serving as a marker of infection. Many defense-related transcripts were persistently down-regulated, including several fibrinogen-containing lectins and homologs of molecules best known from vertebrate phagocytic cells. Our results are consistent with earlier studies suggesting that both parasites are able to interfere with host defense responses, including a tendency for E. paraensei to do so more rapidly and strongly than S. mansoni They further suggest mechanisms for how trematodes are able to establish the chronic infections necessary for their continued success.
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影响因子：
12.3
作者：
Aragon AD;Quiñones GA;Thomas EV;Roy S;Werner-Washburne M
通讯作者：
Werner-Washburne M
影响因子：
4.4
作者：
Barat-Houari M;Hilliou F;Jousset FX;Sofer L;Deleury E;Rocher J;Ravallec M;Galibert L;Delobel P;Feyereisen R;Fournier P;Volkoff AN
通讯作者：
Volkoff AN
影响因子：
6.7
作者：
Bischoff V;Vignal C;Duvic B;Boneca IG;Hoffmann JA;Royet J
通讯作者：
Royet J
影响因子：
1.5
作者：
Goodall, CP;Bender, RC;Bayne, CJ
通讯作者：
Bayne, CJ
影响因子：
2.9
作者：
Guillou, Francois;Mitta, Guillaume;Coustau, Christine
通讯作者：
Coustau, Christine