Gene expression profiling of Spodoptera frugiperda hemocytes and fat body using cDNA microarray reveals polydnavirus-associated variations in lepidopteran host genes transcript levels.
Gene expression profiling of Spodoptera frugiperda hemocytes and fat body using cDNA microarray reveals polydnavirus-associated variations in lepidopteran host genes transcript levels.
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DOI：
10.1186/1471-2164-7-160
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发表时间：
2006-06-21
期刊：
影响因子：
4.4
通讯作者：
Volkoff AN
中科院分区：
文献类型：
--
作者：
Barat-Houari M;Hilliou F;Jousset FX;Sofer L;Deleury E;Rocher J;Ravallec M;Galibert L;Delobel P;Feyereisen R;Fournier P;Volkoff AN
基因组学方法为研究昆虫与病原体的相互作用提供了独特的机会。我们开发了一个cDNA微阵列来分析鳞翅目害虫草地贪夜蛾的基因转录谱响应注射的多DNA病毒HdIV相关的小蜂Hyposoter didyphalus。多脱氧核糖核酸病毒与寄生的姬蜂有关，并且是它们在鳞翅目宿主中发育所必需的，在鳞翅目宿主中它们充当有效的免疫抑制病原体。在这项研究中，我们分析了在两个主要的昆虫免疫反应，血细胞和脂肪体，注射过滤纯化的HdIV后的转录变化。结果显示，注射后24小时，1750个排列的宿主基因中约4%显示其转录水平的变化，其中大部分（76%）显示下降。作为比较，在S.结果表明，在注射致病性JcDNV浓核病毒后，8个基因的转录水平发生了显著变化。它们不同于受HdIV影响的7个，并且与HdIV注射相反，都是上调的。有趣的是，已经显示出由HdIV注射调节的几个基因参与鳞翅目先天免疫。与钙网蛋白，酚氧化酶原激活酶，immulectin-2和一种新的鳞翅目清道夫受体相关的转录水平在HIV注射毛虫的血细胞中降低。这通过定量RT-PCR分析证实，但在注射热灭活的HdIV后未观察到。相反，半乳糖结合凝集素的转录水平增加，令人惊讶的是，酚氧化酶原亚基。所获得的结果表明，HdIV注射影响编码宿主免疫应答（非自我识别、体液和细胞应答）的不同组分的基因的转录水平。通过微阵列方法对宿主-多脱氧核糖核酸病毒相互作用的分析表明，HdIV的存在直接或间接诱导特定宿主基因的转录水平的变化，这些变化可能部分地导致在寄生宿主生理学中观察到的改变。这种全球性的方法的发展将允许更好地了解寄生虫操纵其宿主生理学的策略，并允许识别免疫抑制性多DNA病毒的潜在靶点。
Genomic approaches provide unique opportunities to study interactions of insects with their pathogens. We developed a cDNA microarray to analyze the gene transcription profile of the lepidopteran pest Spodoptera frugiperda in response to injection of the polydnavirus HdIV associated with the ichneumonid wasp Hyposoter didymator. Polydnaviruses are associated with parasitic ichneumonoid wasps and are required for their development within the lepidopteran host, in which they act as potent immunosuppressive pathogens. In this study, we analyzed transcriptional variations in the two main effectors of the insect immune response, the hemocytes and the fat body, after injection of filter-purified HdIV. Results show that 24 hours post-injection, about 4% of the 1750 arrayed host genes display changes in their transcript levels with a large proportion (76%) showing a decrease. As a comparison, in S. frugiperda fat body, after injection of the pathogenic JcDNV densovirus, 8 genes display significant changes in their transcript level. They differ from the 7 affected by HdIV and, as opposed to HdIV injection, are all up-regulated. Interestingly, several of the genes that are modulated by HdIV injection have been shown to be involved in lepidopteran innate immunity. Levels of transcripts related to calreticulin, prophenoloxidase-activating enzyme, immulectin-2 and a novel lepidopteran scavenger receptor are decreased in hemocytes of HdIV-injected caterpillars. This was confirmed by quantitative RT-PCR analysis but not observed after injection of heat-inactivated HdIV. Conversely, an increased level of transcripts was found for a galactose-binding lectin and, surprisingly, for the prophenoloxidase subunits. The results obtained suggest that HdIV injection affects transcript levels of genes encoding different components of the host immune response (non-self recognition, humoral and cellular responses). This analysis of the host-polydnavirus interactions by a microarray approach indicates that the presence of HdIV induces, directly or indirectly, variations in transcript levels of specific host genes, changes that could be responsible in part for the alterations observed in the parasitized host physiology. Development of such global approaches will allow a better understanding of the strategies employed by parasites to manipulate their host physiology, and will permit the identification of potential targets of the immunosuppressive polydnaviruses.
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