The effect of Lactobacillus rhamnosus hsryfm 1301 on the intestinal microbiota of a hyperlipidemic rat model.
The effect of Lactobacillus rhamnosus hsryfm 1301 on the intestinal microbiota of a hyperlipidemic rat model.
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鼠李糖乳杆菌 hsryfm 1301 对高脂血症大鼠模型肠道菌群的影响
DOI：
10.1186/1472-6882-14-386
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发表时间：
2014-10-10
影响因子：
--
通讯作者：
Gu R
中科院分区：
文献类型：
--
作者：
Chen D;Yang Z;Chen X;Huang Y;Yin B;Guo F;Zhao H;Zhao T;Qu H;Huang J;Wu Y;Gu R
背景：越来越多的证据表明，肠道微生物群调节我们的新陈代谢。益生菌带来的健康益处可能取决于它们影响肠道微生物群的能力。本研究的目的是检查补充益生菌菌株鼠李糖乳杆菌hsryfm 1301对高脂血症大鼠模型肠道菌群的影响，并探讨肠道菌群与血脂之间的关系。在L.补充rhamnosus hsryfm 1301或其发酵乳28天。结果：大鼠肠道菌群主要由拟杆菌门、厚壁菌门、变形菌门、螺旋体门和疣微菌门组成。饲喂高脂饲料后，肠道菌群的丰度和多样性普遍下降，拟杆菌门的相对丰度显著下降，厚壁菌门的相对丰度显著增加（P < 0.05）。管理L. rhamnosus hsryfm 1301或其发酵乳作用28 d，可恢复拟杆菌和疣微菌的丰度，降低厚壁菌门的丰度，并显著降低高脂饮食诱导的高脂血症大鼠的血脂水平。益生菌干预28 d后，肠道细菌22个属的丰度发生显著变化（P < 0.05）。Ruminococcus spp.和血清甘油三酯，Dorea spp.和血清胆固醇（TC）和低密度脂蛋白（LDL-C）以及肠球菌属。和高密度脂蛋白。Butyrivibrio spp.结论：补充L.rhamnosus hsryfm 1301或其发酵乳28 d，可通过调节肠道菌群改善高脂血症大鼠的脂代谢。
Background:Growing evidence indicates that intestinal microbiota regulate our metabolism. Probiotics confer health benefits that may depend on their ability to affect the gut microbiota. The objective of this study was to examine the effect of supplementation with the probiotic strain, Lactobacillus rhamnosus hsryfm 1301, on the gut microbiota in a hyperlipidemic rat model, and to explore the associations between the gut microbiota and the serum lipids.Methods:The hyperlipidemic rat model was established by feeding rats a high-fat diet for 28 d. The rats' gut microbiota were analyzed using high-throughput sequencing before and after L. rhamnosus hsryfm 1301 supplementation or its fermented milk for 28 d. The serum lipids level was also tested.Results:The rats' primary gut microbiota were composed of Bacteroidetes, Firmicutes, Proteobacteria, Spirochaetes and Verrucomicrobia. The abundance and diversity of the gut microbiota generally decreased after feeding with a high-fat diet, with a significant decrease in the relative abundance of Bacteroidetes, but with an increase in that of Firmicutes (P < 0.05). Administration of L. rhamnosus hsryfm 1301 or its fermented milk for 28 d, could recover the Bacteroidetes and Verrucomicrobia abundance and could decrease the Firmicutes abundance, which was associated with a significant reduction in the serum lipids' level in the hyperlipidemic rats with high-fat diet induced. The abundance of 22 genera of gut bacteria was changed significantly after probiotic intervention for 28 d (P < 0.05). A positive correlation was observed between Ruminococcus spp. and serum triglycerides, Dorea spp. and serum cholesterol (TC) and low-density lipoprotein (LDL-C), and Enterococcus spp. and high-density lipoprotein. The Butyrivibrio spp. negatively correlated with TC and LDL-C.Conclusions:Our results suggest that the lipid metabolism of hyperlipidemic rats was improved by regulating the gut microbiota with supplementation of L.rhamnosus hsryfm 1301 or its fermented milk for 28 d.
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影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1080/07315724.2007.10719647
发表时间：
2007-12-01
影响因子：
3.5
作者：
Tappenden, Kelly A.;Deutsch, Andrew S.
通讯作者：
Deutsch, Andrew S.
影响因子：
3.6
作者：
Novotny Nunez, Ivanna;Maldonado Galdeano, Carolina;Perdigon, Gabriela
通讯作者：
Perdigon, Gabriela
影响因子：
3.9
作者：
Rubin, Diana;Schneider-Muntau, Alexandra;Doering, Frank
通讯作者：
Doering, Frank
影响因子：
2.8
作者：
Attene-Ramos, Matias S.;Nava, Gerardo M.;Gaskins, H. Rex
通讯作者：
Gaskins, H. Rex