Acceleration Mechanisms of Skin Wound Healing by Autologous Micrograft in Mice.
Acceleration Mechanisms of Skin Wound Healing by Autologous Micrograft in Mice.
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DOI：
10.3390/ijms18081675
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发表时间：
2017-08-02
影响因子：
5.6
通讯作者：
Ohjimi H
中科院分区：
文献类型：
--
作者：
Jimi S;Kimura M;De Francesco F;Riccio M;Hara S;Ohjimi H
最近发展了一种将组织切碎成>50 μm的微碎片的微移植技术。然而，其在创伤愈合中的病理生理机制尚不清楚。因此，我们使用正常小鼠进行了伤口愈合研究。使用具有夹板的皮肤伤口的人源化小鼠模型。在全皮肤切除后，将通过Rigenera方案获得的组织微碎片输注到伤口上。在细胞示踪研究中，使用表达GFP的绿色小鼠和SCID小鼠。胶原染色包括苦味酸天狼星红（PSR）和α-平滑肌肌动蛋白（αSMA）、CD 31、转化生长因子-β1（TGF-β1）和中性粒细胞的免疫组织化学染色评价肉芽组织发育。GFP阳性细胞在输注后7天仍存在于肉芽组织中，但在13天后消失。在将组织微移植物溶液输注到伤口上之后，在早期阶段肉芽组织中的TGF-β1表达瞬时上调。随后，在增厚的肉芽组织中，表达α SMA的肌成纤维细胞数量增加，并加速新生血管形成和胶原基质成熟。在这样的肉芽组织上，再生上皮愈合进展，导致伤口面积减小。微移植物后的替代性改变可能增加了肉芽组织中表达α SMA的肌成纤维细胞，这可能对胶原积聚、新血管形成和伤口收缩起作用。这些变化都有利于创面上皮的再生。
A micrograft technique, which minces tissue into micro-fragments >50 μm, has been recently developed. However, its pathophysiological mechanisms in wound healing are unclear yet. We thus performed a wound healing study using normal mice. A humanized mouse model of a skin wound with a splint was used. After total skin excision, tissue micro-fragments obtained by the Rigenera protocol were infused onto the wounds. In the cell tracing study, GFP-expressing green mice and SCID mice were used. Collagen stains including Picrosirius red (PSR) and immunohistological stains for α-smooth muscle actin (αSMA), CD31, transforming growth factor-β1 (TGF-β1) and neutrophils were evaluated for granulation tissue development. GFP-positive cells remained in granulation tissue seven days after infusion, but vanished after 13 days. Following the infusion of the tissue micrograft solution onto the wound, TGF-β1 expression was transiently upregulated in granulation tissue in the early phase. Subsequently, αSMA-expressing myofibroblasts increased in number in thickened granulation tissue with acceleration of neovascularization and collagen matrix maturation. On such granulation tissue, regenerative epithelial healing progressed, resulting in wound area reduction. Alternative alteration after the micrograft may have increased αSMA-expressing myofibroblasts in granulation tissue, which may act on collagen accumulation, neovascularization and wound contraction. All of these changes are favorable for epithelial regeneration on wound.
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皮肤干细胞作为原始生殖细胞和卵母细胞样细胞的来源
影响因子：
9
作者：
Ge W;Cheng SF;Dyce PW;De Felici M;Shen W
通讯作者：
Shen W
影响因子：
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影响因子：
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作者：
Kessler, D;Dethlefsen, S;Eckes, B
通讯作者：
Eckes, B
影响因子：
5.6
作者：
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通讯作者：
Hara, Shuuji
影响因子：
2.3
作者：
Svolacchia, Fabiano;De Francesco, Francesco;Ferraro, Giuseppe A.
通讯作者：
Ferraro, Giuseppe A.