Effects of dietary fat energy restriction and fish oil feeding on hepatic metabolic abnormalities and insulin resistance in KK mice with high-fat diet-induced obesity.
Effects of dietary fat energy restriction and fish oil feeding on hepatic metabolic abnormalities and insulin resistance in KK mice with high-fat diet-induced obesity.
复制标题
膳食脂肪能量限制和鱼油喂养对高脂饮食肥胖 KK 小鼠肝脏代谢异常和胰岛素抵抗的影响。
DOI：
10.1016/j.jnutbio.2012.06.002
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Akiyo Matsumoto
中科院分区：
文献类型：
--
作者：
Takeshi Arai;Hyoun;Satoshi Hirako;Maki Nakasatomi;H. Chiba;Akiyo Matsumoto
本研究探讨了限制脂肪能量摄入和鱼油摄入对高脂饮食诱导的肥胖雌性KK小鼠糖脂代谢的影响。小鼠喂食猪油/红花油（LSO 50）饲料，其中包含50%能量（en%）猪油/红花油作为脂肪源，持续12周。然后，给小鼠喂食各种脂肪能量限制（25 en%脂肪）饮食-分别含有0、2.5、12.5或25 en%鱼油的LSO、FO 2.5、FO 12.5或FO 25，持续9周。在所有脂肪能量限制组中，无论鱼油含量如何，从HF饮食转换为每种脂肪能量限制饮食均显著降低了最终体重以及内脏和皮下脂肪量。FO12.5和FO 25组的肝脏甘油三酯和胆固醇水平显著降低，但LSO组未降低。虽然血浆胰岛素水平在各组之间没有差异，但口服葡萄糖耐量试验中的血糖曲线下面积在FO12.5和FO 25组中显著较低。实时定量聚合酶链反应分析显示FO 25组脂肪酸合成酶mRNA水平显著降低，FO12.5和FO 25组硬脂酰辅酶A去饱和酶1 mRNA水平显著降低。这些结果表明，即使在HF饮食诱导肥胖的KK小鼠中，饮食脂肪能量限制也抑制了体重增加。我们还认为，脂肪能量限制和鱼油喂养的组合减少了脂肪滴，改善了肝脏肥大和胰岛素抵抗，抑制了这些小鼠的从头脂肪生成。
We investigated the effects of dietary fat energy restriction and fish oil intake on glucose and lipid metabolism in female KK mice with high-fat (HF) diet-induced obesity. Mice were fed a lard/safflower oil (LSO50) diet consisting of 50 energy% (en%) lard/safflower oil as the fat source for 12 weeks. Then, the mice were fed various fat energy restriction (25 en% fat) diets — LSO, FO2.5, FO12.5 or FO25 — containing 0, 2.5, 12.5, or 25 en% fish oil, respectively, for 9 weeks. Conversion from a HF diet to each fat energy restriction diet significantly decreased final body weights and visceral and subcutaneous fat mass in all fat energy restriction groups, regardless of fish oil contents. Hepatic triglyceride and cholesterol levels markedly decreased in the FO12.5 and FO25 groups, but not in the LSO group. Although plasma insulin levels did not differ among groups, the blood glucose areas under the curve in the oral glucose tolerance test were significantly lower in the FO12.5 and FO25 groups. Real-time polymerase chain reaction analysis showed fatty acid synthase mRNA levels significantly decreased in the FO25 group, and stearoyl-CoA desaturase 1 mRNA levels markedly decreased in the FO12.5 and FO25 groups. These results demonstrate that body weight gains were suppressed by dietary fat energy restriction even in KK mice with HF diet-induced obesity. We also suggested that the combination of fat energy restriction and fish oil feeding decreased fat droplets and ameliorated hepatic hypertrophy and insulin resistance with suppression of de novo lipogenesis in these mice.
登录
查看更多内容
DOI：
10.1016/j.bbalip.2008.12.011
发表时间：
2009-02
影响因子：
4.8
作者：
Flowers, Matthew I.;Ntambi, James M.
通讯作者：
Ntambi, James M.
DOI：
10.1172/jci15593
发表时间：
2002-05
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
J. Horton;J. Goldstein;Michael S. Brown
通讯作者：
J. Horton;J. Goldstein;Michael S. Brown
影响因子：
--
作者：
Wilson, PWF;D'Agostino, RB;Kannel, WB
通讯作者：
Kannel, WB
影响因子：
7.1
作者：
Martin, ASG;Obin, MS
通讯作者：
Obin, MS
影响因子：
29
作者：
Miyazaki, Makoto;Flowers, Matthew T.;Ntambi, James M.
通讯作者：
Ntambi, James M.