Agreement Between Eyes in Wide-Field Fluorescence Lifetime Imaging Ophthalmoscopy Measurements at the Human Retina in Healthy Volunteers
Agreement Between Eyes in Wide-Field Fluorescence Lifetime Imaging Ophthalmoscopy Measurements at the Human Retina in Healthy Volunteers
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健康志愿者视网膜的广域荧光寿命成像检眼镜测量中眼睛之间的一致性
DOI：
10.1007/978-981-10-0266-3_63
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
D. Schweitzer
中科院分区：
文献类型：
--
作者：
M. Klemm;E. Nagel;A. Dietzel;K. W. Lai;E. Supriyanto;D. Schweitzer
荧光寿命成像检眼镜（FLIO）是一种新的成像技术，用于测量由眼底内源性荧光团产生的时间分辨自体荧光。目的是评估视网膜的新陈代谢。基于荧光寿命，可以区分眼睛中的不同荧光化合物。病理变化可以通过检测荧光寿命的变化来观察。到目前为止，FLIO使用30°眼底图像进行。对于某些疾病，眼底部分是感兴趣的。在这项工作中，在年轻健康志愿者中进行了观察性临床研究，以检查55°宽视场荧光寿命图像的可用性。测量视网膜自发荧光的时间分辨（扫描激光检眼镜：眼底55°，每像素62 x62 µ m2;激发：皮秒脉冲二极管激光，473 nm，80 MHz重复率;检测：光谱通道498- 560 nm（CH 1）和560- 720 nm（CH 2），时间相关单光子计数法）。在一周内的不同日期，在相似的时间进行了三次重复测量。所有受试者均具有晶状体透镜和未散瞳。应用改进的3指数方法来确定平均荧光寿命tm。在ETDRS网格上，在中央凹上方25°上级的15 × 15像素区域中计算tm。使用Wilcoxon秩和检验来检验左眼和右眼之间的统计学显著性差异。通道1中上级区域的变异系数为5.6%（右）和4.7%（左），通道2中为2.5%（右）和2.1%（左）。右眼和左眼之间未发现统计学显著差异。年轻健康志愿者使用55°透镜进行的FLIO测量在双眼中均是可重复的，并且未显示出显著差异。应首选光谱通道2，因为其可变性较低。数据分析使用FLIMX（可在 http://www.flimx.de ).
Fluorescence lifetime imaging ophthalmoscopy (FLIO) is a new imaging technique for measuring the time-resolved in vivo autofluorescence generated by endogenous fluorophores in the ocular fundus. The aim is to assess the metabolism of the retina. Based on the fluorescence lifetime, different fluorescent compounds in the eye can be distinguished. Pathologic changes may be observed by detecting changes in the fluorescence lifetime. Until now, FLIO is performed using 30° fundus images. For certain diseases section of the fundus is of interest. In this work, observational clinical study in young healthy volunteers was performed to examine the usability of 55° wide-field fluorescence lifetime images. The time-resolved retina autofluorescence was measured (scanning laser ophthalmoscope: 55° of fundus, 62x62 µm2per pixel; excitation: diode laser with pico-second pulses, 473nm, 80MHz repetition rate; detection: spectral channels 498-560nm (ch1) and 560-720nm (ch2), time-correlated single photon counting method) in both eyes of 11 healthy volunteers (28.7±3.6 years). Three repetitive measurements on different days within one week at a similar time have been performed. All subjects had a crystalline lens and an undilated pupil. A modified 3-exponential approach was applied to determine the mean fluorescence lifetimes tm. tmwere computed on the ETDRS grid and in a 15x15 pixel region 25° superior to the fovea. The Wilcoxon rank-sum test was used to test for statistical significant differences between left and right eye. The coefficient of variation in the superior region is 5.6% (right) and 4.7% (left) in ch1 and 2.5% (right) and 2.1% (left) in ch2. No statistical significant differences have been found between right and left eye. FLIO measurements in young healthy volunteers using a 55° lens are repeatable in both eyes and show no significant differences. Spectral channel 2 should be preferred because of its lower variability. The data analysis was done with FLIMX (available at http://www.flimx.de ).
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影响因子：
3.7
作者：
Klemm M;Schweitzer D;Peters S;Sauer L;Hammer M;Haueisen J
通讯作者：
Haueisen J
荧光寿命成像检眼镜检查青光眼
DOI：
--
发表时间：
2014
期刊：
Graefe's Archive for Clinical and Experimental Ophthalmology
影响因子：
--
作者：
L. Ramm;S. Jentsch;R. Augsten;M. Hammer
通讯作者：
M. Hammer
影响因子：
3.5
作者：
Schweitzer, Dietrich;Deutsch, Lydia;Dawczynski, Jens
通讯作者：
Dawczynski, Jens
影响因子：
1.9
作者：
D. Schweitzer;M. Klemm;M. Hammer;S. Jentsch;F. Schweitzer
通讯作者：
F. Schweitzer