Protein Nanofibril Assemblies Templated by Graphene Oxide Nanosheets Accelerate Early Cell Adhesion and Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells.
Protein Nanofibril Assemblies Templated by Graphene Oxide Nanosheets Accelerate Early Cell Adhesion and Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells.
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以氧化石墨烯纳米片为模板的蛋白质纳米纤维组件可加速早期细胞粘附并诱导人间充质干细胞的成骨分化
DOI：
10.1021/acsami.8b11811
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发表时间：
2018-09-26
影响因子：
9.5
通讯作者：
Yang M
中科院分区：
文献类型：
--
作者：
Shuai Y;Mao C;Yang M
家蚕丝素蛋白（SF）是一种很有前途的天然生物相容性蛋白质。然而，其与氧化石墨烯 (GO) 的相互作用从未被研究过，并且所得的 SF/GO 基质尚未用于指导干细胞的命运。在此，我们发现，在水溶液中混合SF分子和GO纳米片，由于GO纳米片的引导，可以触发SF纳米粒子组装成定向纳米纤丝，形成具有独特纳米形貌的SF/GO薄膜，并在去除溶剂后提高模量。当SF/GO薄膜中GO的质量百分比为2%和10%时，所得薄膜中的SF组装体是项链状纳米原纤维（由松散连接的SF纳米粒子组装而成）和固体纳米原纤维（由紧密连接的SF纳米粒子组装而成），分别称为SG2和SG10。傅里叶变换红外光谱和圆二色性测量证实，GO 纳米片通过触发 SF 分子从无规卷曲到 β 片的结构变化，引导 SF 组装成纳米原纤维。此外，根据X射线光电子能谱和拉曼光谱，在纳米纤丝形成过程中，GO纳米片中的氧化基团被SF中的还原基团还原。 SF/GO 薄膜上良好的细胞活力进一步证实了 SF 对 GO 中氧化基团的还原作用。研究发现，即使在培养基中没有额外的诱导剂的情况下，SF/GO 薄膜独特的纳米形貌也能加速早期细胞粘附并诱导人间充质干细胞 (MSC) 的成骨分化。更重要的是，SG10通过促进F-肌动蛋白组装、增加细胞铺展面积以及通过独特的SF/GO纳米纤维基质诱导MSC的成骨分化，在促进早期MSC粘附方面表现出更强的能力。据我们所知，这是首次报道SF/GO底物可以在没有成骨分化培养基的情况下诱导MSCs的成骨分化。因此，SF/GO复合材料在骨组织工程领域具有潜在的应用前景。
Bombyx mori silk fibroin (SF) is a promising natural biocompatible protein. However, its interaction with graphene oxide (GO) has never been studied and the resultant SF/GO matrix has not been used to direct stem cell fate. Herein, we found out that mixing SF molecules and GO nanosheets in an aqueous solution can trigger the assembly of SF nanoparticles into oriented nanofibrils due to the guidance of GO nanosheets, forming SF/GO films with unique nanotopographies and improved modulus upon the removal of the solvent. When GO mass percentage in the SF/GO films is 2 and 10%, the SF assemblies are necklace-like nanofibrils (assembled from loosely linked SF nanoparticles) and solid nanofibrils (assembled from densely linked SF nanoparticles) in the resultant films, termed SG2 and SG10, respectively. GO nanosheets guided the SF assembly into nanofibrils by triggering the structural change of SF molecules from random coils to β-sheets, as confirmed by Fourier transform infrared spectroscopy and circular dichroism measurements. Furthermore, oxidative groups in the GO nanosheets were reduced by the reducing groups in SF during the nanofibril formation according to X-ray photoelectron spectroscopy and Raman spectroscopy. The reduction of the oxidative groups in GO by SF was further verified by the good cell viability on the SF/GO films. The unique nanotopographies of the SF/GO films were found to accelerate the early cell adhesion and induce the osteogenic differentiation of human mesenchymal stem cells (MSCs) even in the absence of additional inducers in the medium. More importantly, SG10 presents a stronger capability in promoting early MSC adhesion by promoting F-actin assembly, increasing cell spreading area, and inducing the osteogenic differentiation of the MSCs by the unique SF/GO nanofibrous matrix. To the best of our knowledge, it is the first report that the SF/GO substrates can induce the osteogenic differentiation of MSCs in the absence of osteogenic differentiation medium. Therefore, SF/GO composite materials would have a potential application in the field of bone tissue engineering.
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影响因子：
16.6
作者：
Cho, Se Youn;Yun, Young Soo;Lee, Sungho;Jang, Dawon;Park, Kyu-Young;Kim, Jae Kyung;Kim, Byung Hoon;Kang, Kisuk;Kaplan, David L.;Jin, Hyoung-Joon
通讯作者：
Jin, Hyoung-Joon
疏水性聚合物薄膜上沉积的自聚合多巴胺涂层的表面特性
影响因子：
3.9
作者：
Jiang, Jinhong;Zhu, Liping;Xu, Youyi
通讯作者：
Xu, Youyi
影响因子：
14
作者：
Ku, Sook Hee;Park, Chan Beum
通讯作者：
Park, Chan Beum
影响因子：
10.9
作者：
Alzhavan, Omid;Ghaderi, Elham;Shahsavar, Mahla
通讯作者：
Shahsavar, Mahla
影响因子：
9.7
作者：
Lu, Qiang;Hu, Xiao;Wang, Xiaoqin;Kluge, Jonathan A.;Lu, Shenzhou;Cebe, Peggy;Kaplan, David L.
通讯作者：
Kaplan, David L.