Microtubule and microfilament rearrangements during capping of concanavalin A receptors on cultured ovarian granulosa cells.
Microtubule and microfilament rearrangements during capping of concanavalin A receptors on cultured ovarian granulosa cells.
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DOI：
10.1083/jcb.73.1.111
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发表时间：
1977-04
期刊：
影响因子：
--
通讯作者：
Anderson E
中科院分区：
文献类型：
--
作者：
Albertini DF;Anderson E
用伴刀豆球蛋白A（Con A）在37 ℃处理的大鼠和家兔培养的颗粒细胞的薄切片电子显微镜分析显示，在帽形成和凝集素受体复合物内化过程中，微丝、粗丝和微管的细胞质分布发生了协调变化。Con A受体聚集伴随着质膜下微丝的积累，这些微丝组装成松散编织的环，因为受体的斑块在细胞表面上集中移动。周期性的密度出现在微丝环中，当斑块合并形成单个中心帽时，微丝环的直径减小。微管和粗丝出现与加盖膜。加帽之后是con A-受体复合物的内吞作用。在此过程中，微丝环被转移到细胞质中，内吞小泡被运输到核旁高尔基复合体沿着微管和粗丝。最后，这些囊泡聚集在细胞中心附近，在那里它们嵌入一个密集的粗丝网络中。冷冻断裂分析的刀豆蛋白A-加盖颗粒细胞的外周膜内颗粒的排列没有改变，但大，光滑的领域是明显的质膜的加盖区域。数据进行了讨论，参照参与的微管和微丝在封盖过程中。
Thin-section electron microscope analysis of rat and rabbit-cultured granulosa cells treated with concanavalin A (Con A) at 37 degrees C revealed coordinated changes in the cytoplasmic disposition of microfilaments, thick filaments, and microtubules during cap formation and internalization of lectin-receptor complexes. Con A-receptor clustering is accompanied by an accumulation of subplasmalemmal microfilaments which assemble into a loosely woven ring as patches of receptor move centrally on the cell surface. Periodic densities appear in the microfilament ring which becomes reduced in diameter as patches coalesce to form a single central cap. Microtubules and thick filaments emerge associated with the capped membrane. Capping is followed by endocytosis of the con A-receptor complexes. During this process, the microfilament ring is displaced basally into the cytoplasm and endocytic vesicles are transported to the paranuclear Golgi complex along microtubules and thick filaments. Eventually, these vesicles aggregate near the cell center where they are embedded in a dense meshwork of thick filaments. Freeze-fracture analysis of Con A-capped granulosa cells revealed no alteration in the arrangement of peripheral intramembrane particles but large, smooth domains were conspicuous in the capped region of the plasma membrane. The data are discussed with reference to the participation of microtubules and microfilaments in the capping process.
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影响因子：
2.7
作者：
ERICKSON, GF;CHALLIS, JRG;RYAN, KJ
通讯作者：
RYAN, KJ
影响因子：
7.8
作者：
GRUENSTEIN, E;RICH, A;WEIHING, RR
通讯作者：
WEIHING, RR
DOI：
10.1073/pnas.71.2.394
发表时间：
1974-01-01
影响因子：
11.1
作者：
OLIVER, JM;UKENA, TE;BERLIN, RD
通讯作者：
BERLIN, RD
影响因子：
56.9
作者：
EDELMAN, GM
通讯作者：
EDELMAN, GM
影响因子：
64.8
作者：
OLIVER, JM;ZURIER, RB;BERLIN, RD
通讯作者：
BERLIN, RD