Transcriptomics analyses reveal that OsMIOX improves rice drought tolerance by regulating the expression of plant hormone and sugar related genes
Transcriptomics analyses reveal that OsMIOX improves rice drought tolerance by regulating the expression of plant hormone and sugar related genes
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转录组学分析表明 OsMIOX 通过调节植物激素和糖相关基因的表达来提高水稻的耐旱性
DOI：
10.1007/s11816-020-00608-7
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发表时间：
2020-02
影响因子：
2.4
通讯作者：
Qiu Dewen
中科院分区：
文献类型：
--
作者：
Shi Fachao;Dong Yijie;Wang Min;Qiu Dewen
干旱胁迫是导致水稻产量和品质下降的主要负因子。肌醇加氧酶（MIOX）是一种独特的单加氧酶，在植物抗旱性中发挥着重要作用。为了研究OsMIOX基因在干旱过程中的功能，通过RNA测序分析了OsMIOX过表达（OE）水稻和野生型（WT）水稻的转录组学特征。在干旱胁迫下，总共产生了2300万个高质量的读数，组装成28,552个unigenes，并且743个基因（534个上调和209个下调）在OE植物和WT之间显著差异表达。这些基因主要与植物激素的传导和糖代谢有关。有趣的是，几个转录因子（TF）显着确定在OE线。RNA-Seq数据和qRT-PCR结果之间存在显著正相关。总之，本研究结果可为鉴定与干旱胁迫相关的候选基因和阐明OsMIOX在干旱胁迫中的作用提供部分有价值的线索。
Drought stress, as a key negative factor, could result in the reduction of yield and quality in rice. Myo-inositol oxygenase (MIOX), a unique monooxygenase, exerts an essential role in plant drought resistance. To investigate the function of the OsMIOX gene in drought process, a transcriptomic profile between OsMIOX-overexpressing (OE) plants and wild-type (WT) was performed by RNA-sequencing in rice. Under drought stress, a total of 23 million high-quality reads, assembled into 28,552 unigenes, were generated and 743 genes (534 up-regulated and 209 down-regulated) were significantly differentially expressed between OE plants and WT. These detected genes were mainly associated with plant hormones transduction and sugar metabolism. Interestingly, several transcription factors (TFs) were prominently identified in OE lines. A significant positive correlation between RNA-Seq data and qRT-PCR results was identified. In all, our results could partially provide relatively valuable clues in identifying candidate genes involved in drought-stress resistance and elucidating basis theory underlying the role of OsMIOX in drought resistance.
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影响因子：
5.7
作者：
Fehlmann T;Reinheimer S;Geng C;Su X;Drmanac S;Alexeev A;Zhang C;Backes C;Ludwig N;Hart M;An D;Zhu Z;Xu C;Chen A;Ni M;Liu J;Li Y;Poulter M;Li Y;Stähler C;Drmanac R;Xu X;Meese E;Keller A
通讯作者：
Keller A
bHLH122 对于拟南芥的干旱和渗透胁迫抵抗以及 ABA 分解代谢的抑制非常重要
影响因子：
9.4
作者：
Liu, Wenwen;Tai, Huanhuan;Li, Wen-Xue
通讯作者：
Li, Wen-Xue
DOI：
10.1126/science.1173041
发表时间：
2009-05-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park SY;Fung P;Nishimura N;Jensen DR;Fujii H;Zhao Y;Lumba S;Santiago J;Rodrigues A;Chow TF;Alfred SE;Bonetta D;Finkelstein R;Provart NJ;Desveaux D;Rodriguez PL;McCourt P;Zhu JK;Schroeder JI;Volkman BF;Cutler SR
通讯作者：
Cutler SR
影响因子：
3.7
作者：
Jisha V;Dampanaboina L;Vadassery J;Mithöfer A;Kappara S;Ramanan R
通讯作者：
Ramanan R
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y