The serotonin 5-HT7Dro receptor is expressed in the brain of Drosophila, and is essential for normal courtship and mating.
The serotonin 5-HT7Dro receptor is expressed in the brain of Drosophila, and is essential for normal courtship and mating.
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DOI：
10.1371/journal.pone.0020800
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Nichols CD
中科院分区：
文献类型：
--
作者：
Becnel J;Johnson O;Luo J;Nässel DR;Nichols CD
5-HT7受体仍然是特性较差的5-羟色胺受体之一。尽管它已被证明参与哺乳动物的情绪、睡眠和昼夜节律的调节，以及血管平滑肌的松弛，但这些功能的确切机制在很大程度上仍不清楚。果蝇，果蝇，是一个有吸引力的模式生物，研究神经药理学，分子和行为过程，在很大程度上是保守的哺乳动物。果蝇表达哺乳动物5-HT7受体的同源物，以及哺乳动物5-HT1a和5-HT2受体的同源物。每个苍蝇受体与它们的哺乳动物受体耦合到相同的效应器途径，并已被证明介导类似的行为反应。在这里，我们报告了5-HT7Dro受体在果蝇中的表达和功能。在幼虫中枢神经系统中，表达在突触后的离散细胞和神经元回路中检测到。在成人大脑中，所有支配椭球体的大视野R神经元以及与PDF阳性的LNvs神经元聚集在一起的一小部分细胞中都有强表达，这些神经元参与了昼夜节律活动。根据药理学和遗传学的方法，我们发现5-HT7Dro的活动对于苍蝇正常的求偶和交配行为是必不可少的，在那里它似乎调节了雄性和雌性的兴趣水平。这是首次有报道的证据表明，一种特定的血清素受体亚型直接参与了苍蝇的求偶和交配。
The 5-HT7 receptor remains one of the less well characterized serotonin receptors. Although it has been demonstrated to be involved in the regulation of mood, sleep, and circadian rhythms, as well as relaxation of vascular smooth muscles in mammals, the precise mechanisms underlying these functions remain largely unknown. The fruit fly, Drosophila melanogaster, is an attractive model organism to study neuropharmacological, molecular, and behavioral processes that are largely conserved with mammals. Drosophila express a homolog of the mammalian 5-HT7 receptor, as well as homologs for the mammalian 5-HT1A, and 5-HT2, receptors. Each fly receptor couples to the same effector pathway as their mammalian counterpart and have been demonstrated to mediate similar behavioral responses. Here, we report on the expression and function of the 5-HT7Dro receptor in Drosophila. In the larval central nervous system, expression is detected postsynaptically in discreet cells and neuronal circuits. In the adult brain there is strong expression in all large-field R neurons that innervate the ellipsoid body, as well as in a small group of cells that cluster with the PDF-positive LNvs neurons that mediate circadian activity. Following both pharmacological and genetic approaches, we have found that 5-HT7Dro activity is essential for normal courtship and mating behaviors in the fly, where it appears to mediate levels of interest in both males and females. This is the first reported evidence of direct involvement of a particular serotonin receptor subtype in courtship and mating in the fly.
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影响因子：
6.1
作者：
FRATTA, W;BIGGIO, G;GESSA, GL
通讯作者：
GESSA, GL
影响因子：
4.7
作者：
Guscott, MR;Egan, E;Hutson, PH
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影响因子：
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作者：
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通讯作者：
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