Altered developmental expression of polymorphic membrane proteins in penicillin-stressed Chlamydia trachomatis.
Altered developmental expression of polymorphic membrane proteins in penicillin-stressed Chlamydia trachomatis.
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DOI：
10.1111/j.1462-5822.2011.01598.x
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发表时间：
2011-07
影响因子：
3.4
通讯作者：
Bavoil PM
中科院分区：
文献类型：
--
作者：
Carrasco JA;Tan C;Rank RG;Hsia RC;Bavoil PM
晚C沙眼包涵体表达表面暴露的多态性膜蛋白家族（Pmp亚型A至I）的每一个成员，其具有完全开启、完全关闭和中间表型的可再现分布。该观察结果与在C中观察到的可变Pmp抗体谱一致。沙眼感染的患者，并导致了这样的假设，即PMP基因家族形成了抗原变异的类似时相变异机制的基础。在这里，我们调查和比较的发育表达的9个pmp基因的条件下，在体外生长的最佳条件下，促进延长生存的衣原体暴露于青霉素诱导的压力。我们证明，pmp基因家族包括不同的转录单位，差异表达沿着发展和差异响应压力。特别是，我们的研究结果表明，pmpA，pmpD和pmpI的表达是独特的不受压力，这表明PmpA，PmpD和PmpI蛋白在C的发病机制中起着关键作用。沙眼
Late C. trachomatis inclusions express each member of the surface-exposed polymorphic membrane protein family (Pmp subtypes A through I) with a reproducible distribution of fully-on, fully-off and intermediate phenotypes. This observation is consistent with observed variable Pmp antibody profiles in C. trachomatis-infected patients and has led to the hypothesis that the pmp gene family forms the basis of a phase variation-like mechanism of antigenic variation. Here we investigate and compare the developmental expression of each of the nine pmp genes under conditions of optimal in vitro growth with that under conditions that promote prolonged survival of chlamydiae when exposed to penicillin-induced stress. We demonstrate that the pmp gene family includes distinct transcriptional units that are differentially expressed along development and differentially responsive to stress. In particular, our results indicate that expression of pmpA, pmpD and pmpI is uniquely unaffected by stress, suggesting that the PmpA, PmpD and PmpI proteins play a critical role in the pathogenesis of C. trachomatis.
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影响因子：
5.8
作者：
Hsia, RC;Ohayon, H;Bavoil, PM
通讯作者：
Bavoil, PM
影响因子：
11.8
作者：
EKMAN, MR;GRAYSTON, JT;SAIKKU, P
通讯作者：
SAIKKU, P
影响因子：
3.2
作者：
ENGEL, JN;POLLACK, J;GANEM, D
通讯作者：
GANEM, D
影响因子：
6.4
作者：
GRAYSTON, JT;ALDOUS, MB;ALTMAN, J
通讯作者：
ALTMAN, J
DOI：
10.1073/pnas.92.15.6911
发表时间：
1995-07-18
影响因子：
11.1
作者：
KUO, CC;GRAYSTON, JT;BENDITT, EP
通讯作者：
BENDITT, EP