Placental inflammation and oxidative stress in the mouse model of assisted reproduction.
Placental inflammation and oxidative stress in the mouse model of assisted reproduction.
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DOI：
10.1016/j.placenta.2011.08.003
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发表时间：
2011-11
期刊：
影响因子：
3.8
通讯作者：
Collier, A. C.
中科院分区：
文献类型：
--
作者：
Raunig, J. M.;Yamauchi, Y.;Ward, M. A.;Collier, A. C.
在采用辅助生殖技术的妊娠中，低出生体重和早产的发生率较高。这两种情况都与胎盘炎症和氧化应激有关。由于孕激素，一种主要的抗炎类固醇，胎盘和胎儿的水平，减少在小鼠ART，我们研究了胎盘炎症和氧化应激在这个模型中作为潜在的介质的负面出生结果。通过体外受精（IVF）和卵胞浆内单精子注射（ICSI）产生小鼠妊娠后，我们评估了母体、胎盘和胎儿组织中的抗氧化防御网络和主要炎症细胞因子。此外，分析胎盘的总脂质水平、纤维化、细胞凋亡、活性氧和细胞内核苷酸的完整性。ART胎盘中脂质含量显著减少，细胞凋亡和核苷酸降解水平更高。ICSI妊娠胎盘的超氧化物歧化酶（SOD）、硫氧还蛋白还原酶（TrxR）、黄嘌呤氧化酶（XO）、过氧化氢酶、谷胱甘肽-S-转移酶（GST）、谷胱甘肽过氧化物酶和谷胱甘肽还原酶（GR）活性较低。ICSI妊娠的胎儿肝脏GR、GST和SOD也较低。IVF妊娠的胎盘仅SOD、TrxR和XO水平降低。在ICSI和IVF妊娠的胎盘中，IL-6水平显着增加。这些数据表明，ART与胎盘炎症（IL-6），氧化应激和细胞凋亡有关，但与纤维化或重塑无关。这些影响在ICSI技术中明显更大。由于ICSI普遍存在，与此方法相关的氧化应激和胎盘炎症可能是导致早产和低出生体重等不良出生结局的关键因素。
Higher rates of low birth weight and prematurity are observed in pregnancies generated with assisted reproduction technologies (ART). Both conditions have been associated with placental inflammation and oxidative stress. Since placental and fetal levels of progesterone, a major anti-inflammatory steroid, are decreased in murine ART, we investigated placental inflammation and oxidative stress in this model as potential mediators of negative birth outcomes. After generating mouse pregnancies by in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) we evaluated the antioxidant defense network and major inflammatory cytokines in maternal, placental and fetal tissues. Additionally, placentas were analyzed for total lipid levels, fibrosis, apoptosis, reactive oxygen species and integrity of intracellular nucleotides. Placentas from ART contained significantly less lipids, with greater levels of apoptosis and degraded nucleotides. Placentas from ICSI pregnancies had lower activities of superoxide dismutase (SOD), thioredoxin reductase (TrxR), xanthine oxidase (XO), catalase, glutathione-S-transferase (GST) glutathione peroxidase, and glutathione reductase (GR). Furthermore, GR, GST and SOD were also lower in fetal livers from ICSI pregnancies. Placentas from IVF pregnancies had decreased levels of SOD, TrxR and XO only. In placentas from both ICSI and IVF pregnancies IL-6 levels were significantly increased. These data suggest that ART is associated with placental inflammation (IL-6), oxidative stress and apoptosis but not fibrosis or remodeling. These effects are markedly greater with the ICSI technique. Since ICSI is ubiquitous, oxidative stress and placental inflammation associated with this method may be a critical factor in negative birth outcomes such as prematurity and low birth weight.
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