An LC-MS/MS method for simultaneous analysis of the cystic fibrosis therapeutic drugs colistin, ivacaftor and ciprofloxacin.
An LC-MS/MS method for simultaneous analysis of the cystic fibrosis therapeutic drugs colistin, ivacaftor and ciprofloxacin.
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DOI：
10.1016/j.jpha.2021.02.004
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发表时间：
2021-12
影响因子：
8.8
通讯作者：
Zhou QT
中科院分区：
文献类型：
--
作者：
Yuan H;Yu S;Chai G;Liu J;Zhou QT
吸入抗生素如粘菌素和环丙沙星越来越多地用于治疗囊性纤维化患者的细菌性肺部感染。本研究建立并验证了一种新的HPLC-MS/MS方法，该方法可以同时检测大鼠血浆、人上皮细胞裂解液、细胞培养基和药物转运介质中的环丙沙星、粘菌素和依伐卡托药物浓度。用600 μL提取液（含0.1%甲酸和0.2%三氟乙酸（TFA）的乙腈）处理200 μL含药物大鼠血浆或细胞培养基的等分试样。加入0.2%TFA有助于破坏药物-蛋白质键。此外，在转运培养基和细胞裂解物样品中加入0.1%甲酸可显著改善响应和重现性。涡旋和振荡后，通过HPLC-MS/MS分析样品组分。使用多反应监测模式检测以下转换：585.5-101.1（粘菌素A），578.5-101.1（粘菌素B）、393.2-337.2（依伐卡托）、332.2-314.2（环丙沙星）、602.3-101.1（多粘菌素B1作为内标物（IS））和595.4-101.1（多粘菌素B2作为IS）。单个样品的运行时间仅为6分钟，使其成为一种省时的方法。多粘菌素A在0.029-5.82 μg/mL、多粘菌素B在0.016-3.14 μg/mL、依伐卡托在0.05-10.0 μg/mL和环丙沙星在0.043-8.58 μg/mL范围内呈线性相关。该方法的准确度、精密度和稳定性均在可接受范围内。该方法对细胞毒性、动物药代动力学和体外药物释放的研究具有重要意义。该方法对于囊性纤维化的细胞毒性、药代动力学和体外药物递送的分析非常有用。该方法是第一个同时分析粘菌素、依伐卡托和环丙沙星的经验证方法。该方法可用于血浆、上皮细胞、细胞培养基和药物转运介质的分析。三氟甲酸作为药物-蛋白质键的断裂剂，加入甲酸以增加药物的反应。该方法可在6 min内检测出3种模型药物。
Inhaled antibiotics such as colistin and ciprofloxacin are increasingly used to treat bacterial lung infections in cystic fibrosis patients. In this study, we established and validated a new HPLC-MS/MS method that could simultaneously detect drug concentrations of ciprofloxacin, colistin and ivacaftor in rat plasma, human epithelial cell lysate, cell culture medium, and drug transport media. An aliquot of 200 μL drug-containing rat plasma or cell culture medium was treated with 600 μL of extraction solution (acetonitrile containing 0.1% formic acid and 0.2% trifluoroacetic acid (TFA)). The addition of 0.2% TFA helped to break the drug-protein bonds. Moreover, the addition of 0.1% formic acid to the transport medium and cell lysate samples could significantly improve the response and reproducibility. After vortexing and centrifuging, the sample components were analyzed by HPLC-MS/MS. The multiple reaction monitoring mode was used to detect the following transitions: 585.5–101.1 (colistin A), 578.5–101.1 (colistin B), 393.2–337.2 (ivacaftor), 332.2–314.2 (ciprofloxacin), 602.3–101.1 (polymyxin B1 as internal standard (IS)) and 595.4–101.1 (polymyxin B2 as IS). The running time of a single sample was only 6 min, making this a time-efficient method. Linear correlations were found for colistin A at 0.029–5.82 μg/mL, colistin B at 0.016–3.14 μg/mL, ivacaftor at 0.05–10.0 μg/mL, and ciprofloxacin at 0.043–8.58 μg/mL. Accuracy, precision, and stability of the method were within the acceptable range. This method would be highly useful for research on cytotoxicity, animal pharmacokinetics, and in vitro drug delivery. The method is highly useful for analysis on cytotoxicity, pharmacokinetics, and in vitro drug delivery for cystic fibrosis. The method is the first validated method for analysis of colistin, ivacaftor and ciprofloxacin simultaneously. The method could be employed in analysis of plasma, epithelial cell, cell culture medium, and drug transport media. Trifluoroformic acid was used as a drug-protein bond cleavage, and formic acid was added to increase the response of drugs. The method could detect the three model drugs within 6 min.
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DOI：
10.1016/j.jpba.2008.12.016
发表时间：
2009-04-05
影响因子：
3.4
作者：
Jansson, Britt;Karvanen, Matti;Friberg, Lena E.
通讯作者：
Friberg, Lena E.
影响因子：
5.8
作者：
Chai, Guihong;Park, Heejun;Zhou, Qi (Tony)
通讯作者：
Zhou, Qi (Tony)
DOI：
10.1016/j.jcf.2014.02.004
发表时间：
2014-09
期刊：
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
影响因子：
--
作者：
Reznikov LR;Abou Alaiwa MH;Dohrn CL;Gansemer ND;Diekema DJ;Stoltz DA;Welsh MJ
通讯作者：
Welsh MJ
影响因子：
3.1
作者：
Caro, Ester;Marce, Rosa M.;Borrull, Francesc
通讯作者：
Borrull, Francesc
影响因子：
4.9
作者：
Buyck, Julien M.;Tulkens, Paul M.;Van Bambeke, Franoise
通讯作者：
Van Bambeke, Franoise