DNA sequence analysis of plasmids from multidrug resistant Salmonella enterica serotype Heidelberg isolates.
DNA sequence analysis of plasmids from multidrug resistant Salmonella enterica serotype Heidelberg isolates.
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DOI：
10.1371/journal.pone.0051160
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Foley SL
中科院分区：
文献类型：
--
作者：
Han J;Lynne AM;David DE;Tang H;Xu J;Nayak R;Kaldhone P;Logue CM;Foley SL
Salmonella enterica serovar Heidelberg is among the most detected serovars in swine and poultry, ranks among the top five serotypes associated with human salmonellosis and is disproportionately associated with invasive infections and mortality in humans. Salmonella are known to carry plasmids associated with antimicrobial resistance and virulence. To identify plasmid-associated genes in multidrug resistant S. enterica serovar Heidelberg, antimicrobial resistance plasmids from five isolates were sequenced using the 454 LifeSciences pyrosequencing technology. Four of the isolates contained incompatibility group (Inc) A/C multidrug resistance plasmids harboring at least eight antimicrobial resistance genes. Each of these strains also carried a second resistance plasmid including two IncFIB, an IncHI2 and a plasmid lacking an identified Inc group. The fifth isolate contained an IncI1 plasmid, encoding resistance to gentamicin, streptomycin and sulfonamides. Some of the IncA/C plasmids lacked the full concert of transfer genes and yet were able to be conjugally transferred, likely due to the transfer genes carried on the companion plasmids in the strains. Several non-IncA/C resistance plasmids also carried putative virulence genes. When the sequences were compared to previously sequenced plasmids, it was found that while all plasmids demonstrated some similarity to other plasmids, they were unique, often due to differences in mobile genetic elements in the plasmids. Our study suggests that Salmonella Heidelberg isolates harbor plasmids that co-select for antimicrobial resistance and virulence, along with genes that can mediate the transfer of plasmids within and among other bacterial isolates. Prevalence of such plasmids can complicate efforts to control the spread of S. enterica serovar Heidelberg in food animal and human populations.
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影响因子：
3.7
作者：
Johnson TJ;Thorsness JL;Anderson CP;Lynne AM;Foley SL;Han J;Fricke WF;McDermott PF;White DG;Khatri M;Stell AL;Flores C;Singer RS
通讯作者：
Singer RS
影响因子：
3.2
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Garcia-Quintanilla, Meritxell;Prieto, Ana I.;Casadesus, Josep
通讯作者：
Casadesus, Josep
影响因子：
4.9
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Johnson, Timothy J.;Wannemeuhler, Yvonne M.;Nolan, Lisa K.
通讯作者：
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影响因子：
3.7
作者：
Darling AE;Mau B;Perna NT
通讯作者：
Perna NT
影响因子：
9.4
作者：
Han, Jing;David, Donna E.;Foley, Steven L.
通讯作者：
Foley, Steven L.