Horizontal gene transfer of a ColV plasmid has resulted in a dominant avian clonal type of Salmonella enterica serovar Kentucky.
Horizontal gene transfer of a ColV plasmid has resulted in a dominant avian clonal type of Salmonella enterica serovar Kentucky.
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DOI：
10.1371/journal.pone.0015524
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发表时间：
2010-12-22
期刊：
影响因子：
3.7
通讯作者：
Singer RS
中科院分区：
文献类型：
--
作者：
Johnson TJ;Thorsness JL;Anderson CP;Lynne AM;Foley SL;Han J;Fricke WF;McDermott PF;White DG;Khatri M;Stell AL;Flores C;Singer RS
肠道沙门氏菌仍然是人类食源性胃肠道疾病的一个重要原因。已从生产禽和零售禽肉中分离出多种沙门氏菌血清型。然而，最近，肠球菌亚种。肯塔基州已成为从肉鸡中分离出来的重要沙门氏菌血清型之一。最近的研究表明，它的出现显然与它获得大肠杆菌毒力质粒的时间一致。在本研究中，我们检测了属于59个不同血清型的902株沙门氏菌是否存在这种质粒。在检测的血清型中，ColV质粒仅在肯塔基（72.9%）、鼠伤寒（15.0%）和海德堡（1.7%）血清型分离株中发现。我们证明了单一的PFGE克隆型肯塔基沙门氏菌含有该质粒，并且肯塔基沙门氏菌获得该质粒显著提高了其在鸡盲肠的定殖能力并引起肠外疾病。对来自不同地理位置、时间点和来源的肯塔基沙门氏菌的三个ColV质粒的完整序列进行比较，发现它们具有几乎相同的遗传结构，且几乎没有单核苷酸变化或插入/缺失。总的来说，ColV质粒似乎是最近由单克隆型肯塔基葡萄球菌获得的，并赋予其宿主增强的定植和适应能力。因此，在家禽中存在从其他肠道细菌向沙门氏菌水平转移毒力和适合度因子的潜在基因，这代表了潜在的人类健康危害。
Salmonella enterica continues to be a significant cause of foodborne gastrointestinal illness in humans. A wide variety of Salmonella serovars have been isolated from production birds and from retail poultry meat. Recently, though, S. enterica subsp. enterica serovar Kentucky has emerged as one of the prominent Salmonella serovars isolated from broiler chickens. Recent work suggests that its emergence apparently coincides with its acquisition of a ColV virulence plasmid. In the present study, we examined 902 Salmonella isolates belonging to 59 different serovars for the presence of this plasmid. Of the serovars examined, the ColV plasmid was found only among isolates belonging to the serovars Kentucky (72.9%), Typhimurium (15.0%) and Heidelberg (1.7%). We demonstrated that a single PFGE clonal type of S. Kentucky harbors this plasmid, and acquisition of this plasmid by S. Kentucky significantly increased its ability to colonize the chicken cecum and cause extraintestinal disease. Comparison of the completed sequences of three ColV plasmids from S. Kentucky isolated from different geographical locales, timepoints and sources revealed a nearly identical genetic structure with few single nucleotide changes or insertions/deletions. Overall, it appears that the ColV plasmid was recently acquired by a single clonal type S. Kentucky and confers to its host enhanced colonization and fitness capabilities. Thus, the potential for horizontal gene transfer of virulence and fitness factors to Salmonella from other enteric bacteria exists in poultry, representing a potential human health hazard.
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影响因子：
4.4
作者：
Johnson, Timothy J.;Wannemuehler, Yvonne;Nolan, Lisa K.
通讯作者：
Nolan, Lisa K.
影响因子：
2
作者：
Lestari, Shofiyah Ika;Han, Feifei;Ge, Beilei
通讯作者：
Ge, Beilei
影响因子：
2.8
作者：
Joerger, Rolf D.;Sartori, Casey A.;Kniel, Kalmia E.
通讯作者：
Kniel, Kalmia E.
DOI：
10.1067/mlc.2002.121550
发表时间：
2002-03-01
期刊：
JOURNAL OF LABORATORY AND CLINICAL MEDICINE
影响因子：
--
作者：
Johnson, JR;Russo, TA
通讯作者：
Russo, TA
影响因子：
64.8
作者：
BINNS, MM;DAVIES, DL;HARDY, KG
通讯作者：
HARDY, KG