Metabolic Alterations in Shrimp Stomach During Acute Hepatopancreatic Necrosis Disease and Effects of Taurocholate on Vibrio parahaemolyticus.
Metabolic Alterations in Shrimp Stomach During Acute Hepatopancreatic Necrosis Disease and Effects of Taurocholate on Vibrio parahaemolyticus.
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DOI：
10.3389/fmicb.2021.631468
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发表时间：
2021
影响因子：
5.2
通讯作者：
Wang HC
中科院分区：
文献类型：
--
作者：
Kumar R;Tung TC;Ng TH;Chang CC;Chen YL;Chen YM;Lin SS;Wang HC
急性肝胰腺坏死病（Acute hepatopancreatic necrosis disease，AHPND）是近年来出现的一种细菌性对虾病，在亚洲许多国家引起了对虾死亡率的上升和经济损失。然而，这种疾病的发病机制的分子因素仍然在很大程度上未知。我们的目的是表征AHPND期间虾胃中的代谢变化，并确定牛磺胆酸盐对引起AHPND的副溶血性弧菌的影响。基于代谢组学，脂质代谢途径和初级胆汁酸（BA）的合成主要影响后AHPND感染。胆汁酸代谢产物，即牛磺胆酸盐，在代谢组学数据库中下调。这促使我们研究牛磺胆酸盐对引起AHPND的副溶血性弧菌中生物膜形成、PirABvp毒素释放和生物膜脱离能力的影响。用高浓度的牛磺胆酸盐（一种主要胆汁酸）处理该细菌，诱导生物膜形成、PirABvp毒素释放并促进细菌细胞的分散。综上所述，我们的研究结果表明，AHPND感染可以影响虾胃中的脂质代谢产物，并进一步表明，初级胆汁酸牛磺胆酸是重要的AHPND引起的副溶血性弧菌的毒力。
Acute hepatopancreatic necrosis disease (AHPND), a recently emerged bacterial shrimp disease, has increased shrimp mortality and caused huge economic losses in many Asian countries. However, molecular factors underlying pathogenesis of this disease remain largely unknown. Our objective was to characterize metabolic alterations in shrimp stomach during AHPND and determine effects of taurocholate on AHPND-causing Vibrio parahaemolyticus. Based on metabolomics, pathways for lipid metabolism and for primary bile acid (BA) synthesis were majorly affected following AHPND infection. Bile acid metabolites, namely taurocholate, were downregulated in the metabolomics database. This prompted us to study effects of taurocholate on biofilm formation, PirABvp toxin release and biofilm detachment capabilities in AHPND-causing V. parahaemolyticus. Treatment of this bacterium with high concentration of taurocholate, a primary bile acid, induced biofilm formation, PirABvp toxin release and facilitated the dispersion of bacterial cells. Taken together, our findings suggest that AHPND infection can affect the lipid metabolites in shrimp stomach, and further suggest that the primary bile acid taurocholate is important for the virulence of AHPND-causing V. parahaemolyticus.
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