Endothelial barrier dysfunction induced by nanoparticle exposure through actin remodeling via caveolae/raft-regulated calcium signalling.
Endothelial barrier dysfunction induced by nanoparticle exposure through actin remodeling via caveolae/raft-regulated calcium signalling.
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DOI：
10.1016/j.impact.2018.02.007
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发表时间：
2018-07
期刊：
影响因子：
4.9
通讯作者：
Doiron AL
中科院分区：
文献类型：
--
作者：
Liu Y;Yoo E;Mahler GJ;Doiron AL
现代纳米技术的快速发展导致纳米材料几乎被用于所有的生活应用中，增加了纳米材料暴露的潜在风险，同时需要设计安全有效的材料。先前的工作已经证明了约20 nm的金纳米颗粒（GNP）对体外内皮屏障功能的特异性作用。为了扩大我们对这种尺寸特异性效应的理解，研究了这种类似尺寸范围内的二氧化钛，二氧化硅和聚苯乙烯纳米颗粒（NP）。发现所有测试的纳米颗粒对细胞活力具有最小的影响，但对内皮屏障功能表现出显著的不利影响。大小范围为20 - 30 nm的纳米颗粒通过小窝/筏介导的内吞作用被内皮细胞内化，在给药后2小时引起细胞内钙升高约30%，并触发肌球蛋白轻链激酶（MLCK）调节的肌动球蛋白收缩。这些效应最终导致20-30 nm范围内所有颗粒类型的内皮单层渗透性增加。这种纳米粒子的渗透诱导的内皮屏障功能障碍可能提供有价值的信息，设计更安全的纳米材料或这种纳米粒子的渗透诱导的渗透性效应在生物医学中的潜在应用。
The rapid development of modern nanotechnology has resulted in nanomaterial being use in nearly all applications of life, raising the potential risk of nanomaterial exposure alongside the need to design safe and effective materials. Previous work has demonstrated a specific effect of gold nanoparticles (GNPs) of approximately 20 nm on endothelial barrier function in vitro. To expand our understanding of this size-specific effect, titanium dioxide, silicon dioxide, and polystyrene nanoparticles (NPs) in this similar size range were studied. All tested nanoparticles were found to have minimal effects on cell viability, but exhibited a significant detrimental effect on endothelial barrier function. Nanoparticles in the size range of 20 to 30 nm were internalized by endothelial cells through caveolae/raft-mediated endocytosis, causing intracellular calcium elevation by approximately 30% at 2 hours after administration, and triggering myosin light chain kinase (MLCK)-regulated actomyosin contraction. These effects culminated in an increase in endothelial monolayer permeability across all particle types within the 20–30 nm range. This nanoparticle exposure-induced endothelial barrier dysfunction may provide valuable information for designing safer nanomaterials or potential applications of this nanoparticle exposure-induced permeability effect in biomedicine.
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影响因子：
10.2
作者：
Barathmanikanth S;Kalishwaralal K;Sriram M;Pandian SR;Youn HS;Eom S;Gurunathan S
通讯作者：
Gurunathan S
DOI：
10.1504/ijbnn.2013.054515
发表时间：
2013
期刊：
International journal of biomedical nanoscience and nanotechnology
影响因子：
--
作者：
Bergin IL;Witzmann FA
通讯作者：
Witzmann FA
影响因子：
11.4
作者：
Limbach, Ludwig K.;Wick, Peter;Stark, Wendelin J.
通讯作者：
Stark, Wendelin J.
影响因子：
2.7
作者：
Darvin, M. E.;Koenig, K.;Lademann, J.
通讯作者：
Lademann, J.
影响因子：
64.8
作者：
BREMEL, RD
通讯作者：
BREMEL, RD