High resolution parallel sequencing reveals multistrain Campylobacter in broiler chicken flocks testing 'negative' by conventional culture methods: implications for control of Campylobacter infection.
High resolution parallel sequencing reveals multistrain Campylobacter in broiler chicken flocks testing 'negative' by conventional culture methods: implications for control of Campylobacter infection.
复制标题
DOI：
10.1016/j.psj.2022.102048
复制
发表时间：
2022-10
期刊：
影响因子：
4.4
通讯作者：
Gebhardt-Henrich, Sabine G.
中科院分区：
文献类型：
--
作者：
Colles, Frances M.;Karasova, Daniela;Crhanova, Magdalena;Preston, Stephen G.;Smith, Adrian L.;Dawkins, Marian S.;Rychlik, Ivan;Gebhardt-Henrich, Sabine G.
受污染的鸡肉是人类弯曲杆菌病的主要来源，尽管努力限制肉鸡(肉)鸡群在农场的定居，但感染率仍然很高。使用常规的培养或qPCR检测方法，通常在3周龄的肉鸡群中检测到弯曲杆菌，导致假设此时感染是水平引入养鸡场。在这项研究中，我们使用由porA基因编码的弯曲杆菌外膜蛋白片段的平行测序来检测从23到28天的肉鸡群收集的新鲜粪便样本中弯曲杆菌DNA的存在。使用PorA靶标测试的所有290个样本中都检测到弯曲杆菌DNA，在使用16S细菌图谱的样本中，48%的样本中检测到弯曲杆菌，无论是否可以使用传统的qPCR阈值检测到弯曲杆菌。一种单一的porAf2变异体在通过常规方法被确定为弯曲菌“阳性”的鸡群中占主导地位，但在弯曲菌“阴性”的鸡群中看到了多样化的模式。要在比常规识别更早的年龄在肉鸡群中常规检测到低水平的弯曲杆菌，需要重新审查如何以及何时最好地将生物安全措施应用于活禽。此外，调查为什么单一的弯曲杆菌变异在一些肉鸡群中繁殖，而不是在其他肉鸡群中繁殖，这可能是有用的。
Contaminated chicken meat is a major source of human Campylobacteriosis and rates of infection remain high, despite efforts to limit the colonisation of broiler (meat) chicken flocks on farms. Using conventional testing methods of culture or qPCR, Campylobacter is typically detected amongst broiler flocks from 3 wk of age, leading to the assumption that infection is introduced horizontally into chicken rearing houses at this time. In this study, we use parallel sequencing of a fragment of the Campylobacter outer membrane protein, encoded by the porA gene, to test for presence of Campylobacter DNA amongst fresh fecal samples collected from broiler flocks aged 23 to 28 d. Campylobacter DNA was detected in all of the 290 samples tested using the porA target, and in 48% of samples using 16S bacterial profiling, irrespective of whether or not Campylobacter could be detected using conventional qPCR thresholds. A single porAf2 variant was predominant among flocks that would be determined to be Campylobacter ‘positive’ by conventional means, but a diverse pattern was seen among flocks that were Campylobacter ‘negative’. The ability to routinely detect low levels of Campylobacter amongst broiler flocks at a much earlier age than would conventionally be identified requires a re-examination of how and when biosecurity measures are best applied for live birds. In addition, it may be useful to investigate why single Campylobacter variants proliferate in some broiler flocks and not others.
登录
查看更多内容
DOI：
10.3390/ani11020568
发表时间：
2021-02-22
期刊：
Animals : an open access journal from MDPI
影响因子：
--
作者：
Gebhardt-Henrich SG;Stratmann A;Dawkins MS
通讯作者：
Dawkins MS
影响因子：
4.2
作者：
Bloomfield SJ;Midwinter AC;Biggs PJ;French NP;Marshall JC;Hayman DTS;Carter PE;Mather AE;Fayaz A;Thornley C;Kelly DJ;Benschop J
通讯作者：
Benschop J
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
--
作者：
SHANNON, CE
通讯作者：
SHANNON, CE
影响因子：
3.7
作者：
Kubasova, Tereza;Kollarcikova, Miloslava;Rychlik, Ivan
通讯作者：
Rychlik, Ivan