Aptamer-functionalized microgel particles for protein detection.
Aptamer-functionalized microgel particles for protein detection.
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DOI：
10.1021/ac202335u
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发表时间：
2011-12-01
影响因子：
7.4
通讯作者：
Doyle, Patrick S.
中科院分区：
文献类型：
--
作者：
Srinivas, Rathi L.;Chapin, Stephen C.;Doyle, Patrick S.
在复杂的生物样品中进行临床相关蛋白的高灵敏度和多路检测对临床蛋白质组学的发展至关重要。近年来，适体已成为蛋白质分析的有用工具，因为它们对蛋白质靶点的特异性和亲和力，以及它们与基于颗粒的检测系统的兼容性。在这项研究中，我们证明了在具有适体捕获序列的编码水凝胶微颗粒上高度敏感地检测人α -凝血酶。我们使用静态成像和微流体流动分析技术来评估夹心分析格式微凝胶的检测能力，该格式利用适体和抗体来报告目标结合事件。缓冲液和试剂浓度进行了优化，以提供最大的反应效率，同时仍然保持一个简单的工作流程，可以很容易地适应其他临床相关蛋白的多路检测。在这项工作中使用的三维、无污垢水凝胶固定支架提供了三对数动态范围，使用单个适体捕获物种的检测极限为4pm，不需要间隔物或信号放大。
Highly sensitive and multiplexed detection of clinically relevant proteins in biologically complex samples is crucial for the advancement of clinical proteomics. In recent years, aptamers have emerged as useful tools for protein analysis due to their specificity and affinity for protein targets as well as their compatibility with particle-based detection systems. In this study, we demonstrate the highly sensitive detection of human alpha-thrombin on encoded hydrogel microparticles functionalized with an aptamer capture sequence. We use static imaging and microfluidic flow-through analysis techniques to evaluate the detection capabilities of the microgels in sandwich assay formats that utilize both aptamers and antibodies for the reporting of target-binding events. Buffers and reagent concentrations were optimized to provide maximum reaction efficiency while still maintaining an assay with a simple workflow that can be easily adapted to the multiplexed detection of other clinically relevant proteins. The three-dimensional, non-fouling hydrogel immobilization scaffold used in this work provides three logs of dynamic range, with a limit of detection of 4 pM using a single aptamer capture species and without the need for spacers or signal amplification.
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DOI：
10.3390/s8021090
发表时间：
2008-02-19
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Hasegawa H;Taira KI;Sode K;Ikebukuro K
通讯作者：
Ikebukuro K
影响因子：
3.8
作者：
Cho, Minseon;Kim, Yeonwha;Ban, Changill
通讯作者：
Ban, Changill
影响因子：
7.4
作者：
Broder, Graham R.;Ranasinghe, Rohan T.;Morgan, Hywel
通讯作者：
Morgan, Hywel
影响因子：
2.5
作者：
AKIYAMA, H;IKEDA, K;MCGEER, PL
通讯作者：
MCGEER, PL
影响因子：
7.4
作者：
Lao, Yeh-Hsing;Peck, Konan;Chen, Lin-Chi
通讯作者：
Chen, Lin-Chi