Multiple anxiogenic drugs recruit a parvalbumin-containing subpopulation of GABAergic interneurons in the basolateral amygdala.
Multiple anxiogenic drugs recruit a parvalbumin-containing subpopulation of GABAergic interneurons in the basolateral amygdala.
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DOI：
10.1016/j.pnpbp.2010.07.012
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发表时间：
2010-10-01
影响因子：
5.6
通讯作者：
Lowry, Christopher A.
中科院分区：
文献类型：
--
作者：
Hale, Matthew W.;Johnson, Philip L.;Westerman, Alex M.;Abrams, Jolane K.;Shekhar, Anantha;Lowry, Christopher A.
基底外侧杏仁核是一个分布和相互连接的网络内的节点结构，调节焦虑状态和焦虑相关行为。多种致焦虑药物的给药增加了细胞反应（即，增加c-Fos表达），但这些细胞的神经化学表型尚不清楚。基底外侧杏仁核含有多巴胺能投射神经元和几个γ-氨基丁酸合成（GABA能）中间神经元群，包括一群共表达兴奋性5-HT 2A受体的表达小清蛋白（PV）的GABA能中间神经元。这些PV表达GABA能中间神经元在焦虑状态中的作用尚不清楚。在本实验中，我们检测了多种抗焦虑药物，包括5-HT 2A/2C受体激动剂间氯苯哌嗪（mCPP）、腺苷受体拮抗剂咖啡因、α2-肾上腺素受体拮抗剂育亨宾和GABAA受体上苯二氮卓别构位点的部分反向激动剂N-甲基-β-咔啉-3-甲酰胺（FG-7142），对基底外侧杏仁核分区PV免疫反应（PV-ir）中间神经元c-Fos表达的影响。除mCPP外，所有药物均增加基底外侧杏仁核前部（BLA）PV阳性神经元中c-Fos的表达。BLA中c-Fos免疫反应（c-Fos-ir）/PV-ir GABA能中间神经元的数量与中缝背核（DR）中c-Fos-ir GABA能神经元的数量以及焦虑相关行为的测量呈正相关。所有四种药物增加c-Fos的表达在非PV-IR细胞在大多数的基底外侧杏仁核的细分取样，与溶剂注射对照组相比。总之，这些数据表明，在基底外侧杏仁核中表达PV/5-HT 2A受体的GABA能中间神经元是调节焦虑状态和焦虑相关行为的DR-基底外侧杏仁核神经元回路的一部分。
The basolateral amygdala is a nodal structure within a distributed and interconnected network that regulates anxiety states and anxiety-related behavior. Administration of multiple anxiogenic drugs increases cellular responses (i.e., increases c-Fos expression) in a subregion of the basolateral amygdala, but the neurochemical phenotypes of these cells are not known. The basolateral amygdala contains glutamatergic projection neurons and several populations of γ-aminobutyric acid-synthesizing (GABAergic) interneurons, including a population of parvalbumin (PV)-expressing GABAergic interneurons that co-express the excitatory 5-HT2A receptor. The role for these PV-expressing GABAergic interneurons in anxiety-states is unclear. In this experiment we examined the effects of multiple anxiogenic drugs including the 5-HT2A/2C receptor agonist m-chlorophenyl piperazine (mCPP), the adenosine receptor antagonist caffeine, the α2-adrenoreceptor antagonist yohimbine and the partial inverse agonist at the benzodiazepine allosteric site on the GABAA receptor, N-methyl-beta-carboline-3-carboxamide (FG-7142), on c-Fos expression in PV-immunoreactive (PV-ir) interneurons in subdivisions of the basolateral amygdala. All drugs with the exception of mCPP increased c-Fos expression in PV-ir neurons in the basolateral amygdaloid nucleus, anterior part (BLA). The numbers of c-Fos-immunoreactive (c-Fos-ir)/PV-ir GABAergic interneurons in the BLA were positively correlated with the numbers of c-Fos-ir serotonergic neurons in the mid-rostrocaudal dorsal raphe nucleus (DR) and with a measure of anxiety-related behavior. All four drugs increased c-Fos expression in non-PV-ir cells in most of the subdivisions of the basolateral amygdala that were sampled, compared with vehicle-injected controls. Together, these data suggest that the PV/5-HT2A receptor expressing GABAergic interneurons in the basolateral amygdala are part of a DR-basolateral amygdala neuronal circuit modulating anxiety-states and anxiety-related behavior.
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影响因子：
10.6
作者：
Christianson, John P.;Ragole, Thomas;Amat, Jose;Greenwood, Benjamin N.;Strong, Paul V.;Paul, Evan D.;Fleshner, Monika;Watkins, Linda R.;Maier, Steven F.
通讯作者：
Maier, Steven F.
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作者：
Clemett, DA;Punhani, T;Fone, KCF
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168.9
作者：
Enoch, MA;Kaye, WH;Goldman, D
通讯作者：
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影响因子：
2.9
作者：
Campbell, BM;Merchant, KM
通讯作者：
Merchant, KM
影响因子：
2.9
作者：
Grahn, RE;Will, MJ;Maier, SF
通讯作者：
Maier, SF