Engineering a Novel Antibody-Peptide Bispecific Fusion Protein Against MERS-CoV
Engineering a Novel Antibody-Peptide Bispecific Fusion Protein Against MERS-CoV
复制标题
设计抗中东呼吸综合征冠状病毒的新型抗体-肽双特异性融合蛋白
作者：
王丽丽;徐吉琰;吴艳玲;应天雷
近年来，通过将两个或多个功能性抗原识别元件组合到单个结构中，在双特异性或多特异性抗体的疗法的工程中做出了巨大的努力。但是，据我们所知
In recent years, tremendous efforts have been made in the engineering of bispecific or multi-specific antibody-based therapeutics by combining two or more functional antigen-recognizing elements into a single construct. However, to the best of our knowledge there has been no reported cases of effective antiviral antibody-peptide bispecific fusion proteins. We previously developed potent fully human monoclonal antibodies and inhibitory peptides against Middle East Respiratory Syndrome Coronavirus (MERS-CoV), a novel coronavirus that causes severe acute respiratory illness with high mortality. Here, we describe the generation of antibody-peptide bispecific fusion proteins, each of which contains an anti-MERS-CoV single-chain antibody m336 (or normal human IgG1 CH3 domain as a control) linked with, or without, a MERS-CoV fusion inhibitory peptide HR2P. We found that one of these fusion proteins, designated as m336 diabody-pep, exhibited more potent inhibitory activity than the antibody or the peptide alone against pseudotyped MERS-CoV infection and MERS-CoV S protein-mediated cell-cell fusion, suggesting its potential to be developed as an effective bispecific immunotherapeutic for clinical use.
登录
查看更多内容
DOI：
10.1016/j.jinf.2012.10.002
发表时间：
2012-12
期刊：
The Journal of infection
影响因子：
--
作者：
Chan JF;Li KS;To KK;Cheng VC;Chen H;Yuen KY
通讯作者：
Yuen KY
影响因子：
7.6
作者：
van Doremalen N;Falzarano D;Ying T;de Wit E;Bushmaker T;Feldmann F;Okumura A;Wang Y;Scott DP;Hanley PW;Feldmann H;Dimitrov DS;Munster VJ
通讯作者：
Munster VJ
影响因子：
4.7
作者：
Wang, Cong;Hua, Chen;Jiang, Shibo
通讯作者：
Jiang, Shibo
影响因子：
5.4
作者：
Cockrell, Adam S.;Peck, Kayla M.;Heise, Mark T.
通讯作者：
Heise, Mark T.
一种有效的种系样人单克隆抗体靶向 H7N9 流感血凝素上的 pH 敏感表位
影响因子：
30.3
作者：
Yu F;Song H;Wu Y;Chang SY;Wang L;Li W;Hong B;Xia S;Wang C;Khurana S;Feng Y;Wang Y;Sun Z;He B;Hou D;Manischewitz J;King LR;Song Y;Min JY;Golding H;Ji X;Lu L;Jiang S;Dimitrov DS;Ying T
通讯作者：
Ying T