Hijacking of transcriptional condensates by endogenous retroviruses.
Hijacking of transcriptional condensates by endogenous retroviruses.
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DOI：
10.1038/s41588-022-01132-w
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发表时间：
2022-08
期刊：
影响因子：
30.8
通讯作者：
Hnisz, Denes
中科院分区：
文献类型：
--
作者：
Asimi, Vahid;Kumar, Abhishek Sampath;Niskanen, Henri;Riemenschneider, Christina;Hetzel, Sara;Naderi, Julian;Fasching, Nina;Popitsch, Niko;Du, Manyu;Kretzmer, Helene;Smith, Zachary D.;Weigert, Raha;Walther, Maria;Mamde, Sainath;Meierhofer, David;Wittler, Lars;Buschow, Rene;Timmermann, Bernd;Cisse, Ibrahim I.;Ameres, Stefan L.;Meissner, Alexander;Hnisz, Denes
Most endogenous retroviruses (ERVs) in mammals are incapable of retrotransposition; therefore, why ERV derepression is associated with lethality during early development has been a mystery. Here, we report that rapid and selective degradation of the heterochromatin adapter protein TRIM28 triggers dissociation of transcriptional condensates from loci encoding super-enhancer (SE)-driven pluripotency genes and their association with transcribed ERV loci in murine embryonic stem cells. Knockdown of ERV RNAs or forced expression of SE-enriched transcription factors rescued condensate localization at SEs in TRIM28-degraded cells. In a biochemical reconstitution system, ERV RNA facilitated partitioning of RNA polymerase II and the Mediator coactivator into phase-separated droplets. In TRIM28 knockout mouse embryos, single-cell RNA-seq analysis revealed specific depletion of pluripotent lineages. We propose that coding and noncoding nascent RNAs, including those produced by retrotransposons, may facilitate ‘hijacking’ of transcriptional condensates in various developmental and disease contexts. TRIM28 depletion in embryonic stem cells disconnects transcriptional condensates from super-enhancers, which is rescued by knockdown of endogenous retroviruses.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
64.5
作者：
Hnisz D;Abraham BJ;Lee TI;Lau A;Saint-André V;Sigova AA;Hoke HA;Young RA
通讯作者：
Young RA
影响因子：
7.7
作者：
Cho, Won-Ki;Jayanth, Namrata;Cisse, Ibrahim I.
通讯作者：
Cisse, Ibrahim I.
影响因子：
4.8
作者：
Gilles, Jean-Francois;Dos Santos, Marc;Heck, Nicolas
通讯作者：
Heck, Nicolas
影响因子：
16.8
作者：
Fadloun, Anas;Le Gras, Stephanie;Torres-Padilla, Maria-Elena
通讯作者：
Torres-Padilla, Maria-Elena