Deep metaproteomic analysis of human salivary supernatant.
Deep metaproteomic analysis of human salivary supernatant.
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DOI：
10.1002/pmic.201100503
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发表时间：
2012-04
期刊：
影响因子：
3.4
通讯作者：
Rudney, Joel D.
中科院分区：
文献类型：
--
作者：
Jagtap, Pratik;McGowan, Thomas;Bandhakavi, Sricharan;Tu, Zheng Jin;Seymour, Sean;Griffin, Timothy J.;Rudney, Joel D.
人类唾液蛋白质组极其复杂，包括唾液腺、血清和口腔微生物的蛋白质。人们对宿主成分了解很多，但对微生物成分知之甚少。在这里，我们报告了一个荟萃蛋白质组学分析唾液上清液汇集6名健康受试者。对于唾液蛋白质组的深入询问，我们结合蛋白质动态范围压缩，多维肽分馏，和高质量精度MS/MS与一种新的两步肽鉴定方法，使用人类蛋白质数据库加上从口腔微生物基因组翻译的那些。从124种微生物以及未培养的微生物类型如TM 7中鉴定出肽。链球菌属、罗氏菌属、放线菌属、普雷沃菌属、奈瑟菌属、韦荣氏菌属、乳杆菌属、月形单胞菌属、假单胞菌属、葡萄球菌属和弯曲杆菌属等12门65个属的细菌数量较多。我们研究中的分类多样性与唾液的宏基因组研究大致一致。蛋白质映射到二十个KEGG途径，其中碳水化合物代谢，氨基酸代谢，能量代谢，翻译，膜转运和信号转导最具代表性。取样的群落似乎积极参与糖酵解和蛋白质合成。这是第一个来自人类唾液上清液的深层元蛋白质组学目录，为未来研究微生物多样性和蛋白质活性变化提供了基线，这些变化可能与口腔疾病相关。
The human salivary proteome is extremely complex, including proteins from salivary glands, serum, and oral microbes. Much has been learned about the host component, but little is known about the microbial component. Here we report a metaproteomic analysis of salivary supernatant pooled from 6 healthy subjects. For deep interrogation of the salivary proteome, we combined protein dynamic range compression, multidimensional peptide fractionation, and high mass accuracy MS/MS with a novel two-step peptide identification method using a database of human proteins plus those translated from oral microbe genomes. Peptides were identified from 124 microbial species as well as uncultured phylotypes such as TM7. Streptococcus, Rothia, Actinomyces, Prevotella, Neisseria, Veilonella, Lactobacillus, Selenomonas, Pseudomonas, Staphylococcus, and Campylobacter were abundant among the 65 genera from 12 phyla represented. Taxonomic diversity in our study was broadly consistent with metagenomic studies of saliva. Proteins mapped to twenty KEGG pathways, with Carbohydrate Metabolism, Amino Acid Metabolism, Energy Metabolism, Translation, Membrane Transport, and Signal Transduction most represented. The communities sampled appear to be actively engaged in glycolysis and protein synthesis. This first deep metaproteomic catalog from human salivary supernatant provides a baseline for future studies of shifts in microbial diversity and protein activities potentially associated with oral disease.
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影响因子：
4.2
作者：
Kesimer M;Kiliç N;Mehrotra R;Thornton DJ;Sheehan JK
通讯作者：
Sheehan JK
影响因子：
3.6
作者：
Ling, Zongxin;Kong, Jianming;Xiang, Charlie
通讯作者：
Xiang, Charlie
DOI：
10.1038/ismej.2010.30
发表时间：
2010-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1016/j.jasms.2009.05.007
发表时间：
2009-08-01
影响因子：
3.2
作者：
Cox, Juergen;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
3.1
作者：
Cole, JN;Ramirez, RD;Walker, MJ
通讯作者：
Walker, MJ