Bacterial diversity in the oral cavity of 10 healthy individuals.
Bacterial diversity in the oral cavity of 10 healthy individuals.
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DOI：
10.1038/ismej.2010.30
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发表时间：
2010-08
期刊：
影响因子：
--
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中科院分区：
文献类型：
--
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采用非培养技术测定了10例健康口腔组织的口腔微生物群组成。从每个个体中，在同一时间点从不同的口腔部位收集并汇总了26个标本。利用所有10个个体的部分龈下标本构建了第11个池。利用广泛的细菌引物扩增16S rRNA基因，并从个体和龈下池中构建克隆文库。从11 368个高质量、非嵌合、接近全长的序列中，鉴定出247个物种水平的种型（使用99%的序列识别阈值）和9个细菌门。在所有10个个体中至少保守了15个细菌属，在种和菌株水平上存在显著的个体间差异。将这些口腔细菌序列与之前在其他健康个体的大肠和粪便中发现的接近全长的序列进行比较，表明口腔和肠道含有不同的细菌组。共现分析表明，群落成员在属水平上存在显著的分离，而在种水平上则不存在，这表明生态显著的竞争相互作用在更广泛的分类水平上比在种水平上更为明显。这项研究是迄今为止对健康人类口腔内细菌多样性进行的较全面、高分辨率分析之一，并突出了宏观生态学工具对增强我们对人类健康细菌生态学的理解的价值。
The composition of the oral microbiota from 10 individuals with healthy oral tissues was determined using culture-independent techniques. From each individual, 26 specimens, each from different oral sites at a single point in time, were collected and pooled. An eleventh pool was constructed using portions of the subgingival specimens from all 10 individuals. The 16S rRNA gene was amplified using broad-range bacterial primers, and clone libraries from the individual and subgingival pools were constructed. From a total of 11 368 high-quality, non-chimeric, near full-length sequences, 247 species-level phylotypes (using a 99% sequence identity threshold) and 9 bacteria phyla were identified. At least 15 bacterial genera were conserved among all 10 individuals, with significant interindividual differences at the species and strain level. Comparisons of these oral bacterial sequences to near full-length sequences found previously in the large intestines and feces of other healthy individuals suggest that the mouth and intestinal tract harbor distinct sets of bacteria. Co-occurrence analysis demonstrated significant segregation of taxa when community membership was examined at the level of genus, but not at the level of species, suggesting that ecologically-significant, competitive interactions are more apparent at a broader taxonomic level than species. This study is one of the more comprehensive, high-resolution analyses of bacterial diversity within the healthy human mouth to date, and highlights the value of tools from macroecology for enhancing our understanding of bacterial ecology in human health.
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DOI：
10.1073/pnas.0308766101
发表时间：
2004-04-20
影响因子：
11.1
作者：
Lepp, PW;Brinig, MM;Relman, DA
通讯作者：
Relman, DA
影响因子：
8.8
作者：
Chao, A;Chazdon, RL;Shen, TJ
通讯作者：
Shen, TJ
影响因子：
3
作者：
Lozupone C;Hamady M;Knight R
通讯作者：
Knight R
影响因子：
9.4
作者：
Kazor, CE;Mitchell, PM;Paster, BJ
通讯作者：
Paster, BJ
影响因子：
9.4
作者：
Kumar, PS;Griffen, AL;Leys, EJ
通讯作者：
Leys, EJ