Yeast cell cycle protein CDC48p shows full-length homology to the mammalian protein VCP and is a member of a protein family involved in secretion, peroxisome formation, and gene expression.
Yeast cell cycle protein CDC48p shows full-length homology to the mammalian protein VCP and is a member of a protein family involved in secretion, peroxisome formation, and gene expression.
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DOI：
10.1083/jcb.114.3.443
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发表时间：
1991-08
期刊：
影响因子：
--
通讯作者：
Mecke D
中科院分区：
文献类型：
--
作者：
Fröhlich KU;Fries HW;Rüdiger M;Erdmann R;Botstein D;Mecke D
细胞周期基因cdc 48 -1的酵母突变体表现为大的出芽细胞，微管从单个纺锤体空斑异常地散布在整个细胞质中。该基因被克隆，破坏证明它是必要的。CDC 48序列编码92 kD的蛋白质，该蛋白质具有200个氨基酸的内部重复，并包括核苷酸结合共有序列。脊椎动物VCP在整个蛋白质长度上具有70%的同一性。酵母Sec 18 p和哺乳动物N-乙基马来酰亚胺敏感性融合蛋白（参与细胞内转运）、酵母Pas 1 p（过氧化物酶体组装所必需）和哺乳动物TBP-1（影响HIV基因表达）在重复区域中有40%相同。针对CDC 48的抗体识别显然为115 kD的酵母蛋白和100 kD的哺乳动物蛋白。这两种蛋白质松散地结合到微粒体部分的组分，如Sec 18 p和N-乙基马来酰亚胺敏感性融合蛋白所述。这种相似性表明，CDC 48 p参与细胞周期功能相关的N-乙基马来酰亚胺敏感的融合蛋白/Sec 18 p在高尔基体运输。
Yeast mutants of cell cycle gene cdc48-1 arrest as large budded cells with microtubules spreading aberrantly throughout the cytoplasm from a single spindle plaque. The gene was cloned and disruption proved it to be essential. The CDC48 sequence encodes a protein of 92 kD that has an internal duplication of 200 amino acids and includes a nucleotide binding consensus sequence. Vertebrate VCP has a 70% identity over the entire length of the protein. Yeast Sec18p and mammalian N- ethylmaleimide-sensitive fusion protein, which are involved in intracellular transport, yeast Pas1p, which is essential for peroxisome assembly, and mammalian TBP-1, which influences HIV gene expression, are 40% identical in the duplicated region. Antibodies against CDC48 recognize a yeast protein of apparently 115 kD and a mammalian protein of 100 kD. Both proteins are bound loosely to components of the microsomal fraction as described for Sec18p and N-ethylmaleimide- sensitive fusion protein. This similarity suggests that CDC48p participates in a cell cycle function related to that of N- ethylmaleimide-sensitive fusion protein/Sec18p in Golgi transport.
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影响因子：
11.4
作者：
CELLINI, A;FELDER, E;ROSSI, JJ
通讯作者：
ROSSI, JJ
DOI：
10.1073/pnas.83.4.907
发表时间：
1986-02-01
影响因子：
11.1
作者：
FRY, DC;KUBY, SA;MILDVAN, AS
通讯作者：
MILDVAN, AS
影响因子：
14.9
作者：
BARKER, DG;WHITE, JHM;JOHNSTON, LH
通讯作者：
JOHNSTON, LH
DOI：
10.1073/pnas.83.6.1622
发表时间：
1986-03-01
影响因子：
11.1
作者：
DUNPHY, WG;PFEFFER, SR;ROTHMAN, JE
通讯作者：
ROTHMAN, JE
DOI：
10.1073/pnas.82.11.3756
发表时间：
1985-01-01
影响因子：
11.1
作者：
CARLE, GF;OLSON, MV
通讯作者：
OLSON, MV