Genome-wide association mapping identifies yellow rust resistance loci in Ethiopian durum wheat germplasm.
Genome-wide association mapping identifies yellow rust resistance loci in Ethiopian durum wheat germplasm.
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全基因组的关联映射标识了埃塞俄比亚硬质小麦种质中的黄色锈蚀位点。
DOI：
10.1371/journal.pone.0243675
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Uauy C
中科院分区：
文献类型：
--
作者：
Alemu SK;Huluka AB;Tesfaye K;Haileselassie T;Uauy C
硬粒小麦是埃塞俄比亚种植的一种重要谷物，该国也是其遗传多样性的中心。由小麦条锈病引起的黄（条）锈病是威胁埃塞俄比亚小麦生产的最具破坏性的病害之一。为确定黄锈病的遗传抗性来源，对300个硬粒小麦品种（包括261个地方品种和39个栽培品种）的抗黄锈性进行了全基因组关联研究。在2015年和2016年的主要生长期，使用改良的Cobb量表对Meraro、Kulumsa和cheefe Donsa的材料进行田间抗性评价。对该群体的35K Axiom Array基因分型数据进行分析，共获得8797个多态性snp，其中7093个用于后续分析。种群结构分析表明两个类群中栽培品种与地方品种有明显的区别。在4条染色体（1A、1B、2B和5A）上鉴定出11个与黄锈病抗性显著相关的snp，这些snp定义了至少5个基因组位点。6个snp在1B染色体上被一致地鉴定出来，在每个和组合的整体环境中，解释了62.6-64.0%的表型变异（R2）。抗性等位基因频率范围为14.0 ~ 71.0%；放大已鉴定的抗性位点，发现存在与植物防御系统相关的抗病相关基因，如ABC转运蛋白家族、抗病蛋白RPM1 （NBS-LRR类）、受体激酶和蛋白激酶。该研究为追踪小麦抗黄锈相关位点提供了SNPs，并为其他重要农艺性状的关联研究提供了多样性面板。
Durum wheat is an important cereal grown in Ethiopia, a country which is also its center for genetic diversity. Yellow (stripe) rust caused by Puccinia striiformis fsp tritici is one of the most devastating diseases threatening Ethiopian wheat production. To identify sources of genetic resistance and combat this pathogen, we conducted a genome wide association study of yellow rust resistance on 300 durum wheat accessions comprising 261 landraces and 39 cultivars. The accessions were evaluated for their field resistance using a modified Cobb scale at Meraro, Kulumsa and Chefe Donsa in the 2015 and 2016 main growing seasons. Analysis of the 35K Axiom Array genotyping data of the panel resulted in a total of 8,797 polymorphic SNPs of which 7,093 were used in subsequent analyses. Population structure analysis suggested two groups in which the cultivars clearly stood out separately from the landraces. Eleven SNPs significantly associated with yellow rust resistance were identified on four chromosomes (1A, 1B, 2B, and 5A) which defined at least five genomic loci. Six of the SNPs were consistently identified on chromosome 1B singly at each and combined overall environments which explained 62.6–64.0% of the phenotypic variation (R2). Resistant allele frequency ranged from 14.0–71.0%; Zooming in to the identified resistance loci revealed the presence of disease resistance related genes involved in the plant defense system such as the ABC transporter gene family, disease resistance protein RPM1 (NBS-LRR class), Receptor kinases and Protein kinases. This study has provided SNPs for tracking the loci associated with yellow rust resistance and a diversity panel which can be used for association study of other agriculturally important traits in durum wheat.
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影响因子：
2.7
作者：
Bechere, E;Belay, G;Merker, A
通讯作者：
Merker, A
影响因子：
10.7
作者：
Kilian, B.;Ozkan, H.;Salamini, F.
通讯作者：
Salamini, F.
影响因子：
1.9
作者：
BELAY, G;TESEMMA, T;MERKER, A
通讯作者：
MERKER, A
影响因子：
5.6
作者：
Kidane YG;Hailemariam BN;Mengistu DK;Fadda C;Pè ME;Dell'Acqua M
通讯作者：
Dell'Acqua M
影响因子：
3
作者：
Lawrence R;Day-Williams AG;Mott R;Broxholme J;Cardon LR;Zeggini E
通讯作者：
Zeggini E