Genome-Wide Association Study of Septoria tritici Blotch Resistance in Ethiopian Durum Wheat Landraces.
Genome-Wide Association Study of Septoria tritici Blotch Resistance in Ethiopian Durum Wheat Landraces.
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DOI：
10.3389/fpls.2017.01586
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发表时间：
2017
影响因子：
5.6
通讯作者：
Dell'Acqua M
中科院分区：
文献类型：
--
作者：
Kidane YG;Hailemariam BN;Mengistu DK;Fadda C;Pè ME;Dell'Acqua M
黑穗病（STB）是一种严重影响硬粒小麦和面包小麦种植的真菌病害。小麦抗性遗传物质的鉴定、开发和利用是克服杀菌剂处理成本和局限性的关键。在未开发的遗传物质中寻找抗性来源可能会加速在田间部署STB遗传抗性。埃塞俄比亚的硬粒小麦地方品种代表了这种多样性的宝贵来源。在这项研究中，318个埃塞俄比亚硬粒小麦基因型，其中大部分是传统的地方品种，对STB感染的不同方面的抗性进行了表型分析。同时测定物候、产量和产量组成性状。本文介绍了现代品种和地方品种抗STB性状的分布，以及与其他农艺性状的关系。在地方品种和现代品种中都发现了STB抗性来源，这表明存在与育种相关的等位基因。利用超过1.6万个全基因组多态性标记对遗传物质进行基因分型，以描述基因型组中存在的连锁不平衡和遗传结构，并进行全基因组关联（GWA）研究，以确定与STB抗性相关的基因组位点。面板的高多样性和低遗传结构使得GWA效率高。GWA扫描检测到5个主要的假定的STB抗性QTL，仅部分与小麦文献中已经报道的QTL重叠。我们报告了四个推测的Septoria抗性位点，在以前的文献中没有匹配：两个在Chr 3A和5A上非常重要，两个在Chr 4B和5B上暗示。这些QTL背后的标记解释了高达10%的抗病表型变异。我们发现有3个农艺性状的QTL与STB GWA衍生的标记性状关联重叠。本研究结果表明，埃塞俄比亚未开发的等位基因多样性对研究STB抗性的分子基础以及在当地和国际材料中进行抗性育种具有重要价值。
Septoria tritici blotch (STB) is a devastating fungal disease affecting durum and bread wheat cultivation worldwide. The identification, development, and employment of resistant wheat genetic material is the key to overcoming costs and limitations of fungicide treatments. The search for resistance sources in untapped genetic material may speed up the deployment of STB genetic resistance in the field. Ethiopian durum wheat landraces represent a valuable source of such diversity. In this study, 318 Ethiopian durum wheat genotypes, for the most part traditional landraces, were phenotyped for resistance to different aspects of STB infection. Phenology, yield and yield component traits were concurrently measured the collection. Here we describe the distribution of STB resistance traits in modern varieties and in landraces, and the relation existing between STB resistance and other agronomic traits. STB resistance sources were found in landraces as well as in modern varieties tested, suggesting the presence of alleles of breeding relevance. The genetic material was genotyped with more than 16 thousand genome-wide polymorphic markers to describe the linkage disequilibrium and genetic structure existing within the panel of genotypes, and a genome-wide association (GWA) study was run to allow the identification of genomic loci involved in STB resistance. High diversity and low genetic structure in the panel allowed high efficiency GWA. The GWA scan detected five major putative QTL for STB resistance, only partially overlapping those already reported in the wheat literature. We report four putative loci for Septoria resistance with no match in previous literature: two highly significant ones on Chr 3A and 5A, and two suggestive ones on Chr 4B and 5B. Markers underlying these QTL explained as much as 10% of the phenotypic variance for disease resistance. We found three cases in which putative QTL for agronomic traits overlapped marker trait association deriving from STB GWA. Our results show that the Ethiopian untapped allelic diversity bears a great value in studying the molecular basis of STB resistance and in breeding for resistance in local and international material.
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DOI：
10.1016/j.fgb.2015.04.017
发表时间：
2015-06
期刊：
Fungal genetics and biology : FG & B
影响因子：
--
作者：
Brown JK;Chartrain L;Lasserre-Zuber P;Saintenac C
通讯作者：
Saintenac C
影响因子：
5.4
作者：
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Kema, Gert H. J.
影响因子：
5.4
作者：
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通讯作者：
Goodwin, SB
影响因子：
2.7
作者：
Chartrain, L.;Sourdille, P.;Brown, J. K. M.
通讯作者：
Brown, J. K. M.
影响因子：
5.4
作者：
Eriksen, L;Borum, F;Jahoor, A
通讯作者：
Jahoor, A