Plasma DNA microsatellites as tumor-specific markers and indicators of tumor progression in melanoma patients.
Plasma DNA microsatellites as tumor-specific markers and indicators of tumor progression in melanoma patients.
复制标题
血浆 DNA 微卫星作为黑色素瘤患者肿瘤特异性标记物和肿瘤进展指标。
作者：
Y. Fujiwara;D. Chi;He‐jing Wang;Pond Keleman;D. Morton;R. Turner;D. Hoon
在黑色素瘤中存在多个DNA微卫星，并伴有频繁的杂合性丢失（洛）。游离DNA在黑色素瘤患者血液中富集的发现促使研究确定肿瘤特异性DNA，如表现出洛的DNA微卫星，是否可以在血液中检测到并具有临床用途。在这项研究中，57名晚期和19名早期临床分期的黑色素瘤患者使用6条染色体上的10个微卫星标记进行了评估。40例患者的血浆和黑素瘤组织中的洛缺失（LOH）均呈高度一致性（P < 0.0001）。血浆中检测到的洛微卫星标记的频率在更晚期的患者中显著增加。在D3 S1293位点，洛杂合性缺失检测与临床疾病进展显著相关（P = 0.02）。此外，D9S157和D3S1293（P = 0.01）、D9S157和D1S228（P = 0.05）、D11S925和D3S1293（P = 0.01）的组合与疾病的不同临床阶段的进展显著相关。这些研究表明，血浆中的肿瘤特异性洛缺失标记物作为黑色素瘤患者的诊断和预后标记物具有潜在的临床用途。
Multiple DNA microsatellites with frequent loss of heterozygosity (LOH) in melanomas have been demonstrated. The finding that free DNA is enriched in blood of melanoma patients prompted studies to determine whether tumor-specific DNA, such as DNA microsatellites exhibiting LOH, can be detected in blood and have clinical use. In this study, 57 advanced and 19 early clinically staged melanoma patients were assessed using 10 microsatellite markers on six chromosomes. Matched plasma and melanoma tissues from 40 patients showed significant concordance of LOH (P < 0.0001). The frequency of LOH microsatellite markers detected in plasma significantly increased in more advanced-staged patients. At locus D3S1293, LOH detection showed significant correlation to clinical disease progression (P = 0.02). Additionally, the combination of LOH microsatellite markers D9S157 and D3S1293 (P = 0.01), D9S157 and D1S228 (P = 0.05), and D11S925 and D3S1293 (P = 0.01) were significantly correlated to progression of different clinical stages of disease. These studies indicate that tumor-specific LOH markers in plasma have a potential clinical use as diagnostic and prognostic markers in melanoma patients.
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影响因子：
11.2
作者：
T. Sarantou;D. Chi;D. Garrison;A. Conrad;P. Schmid;D. Morton;D. Hoon
通讯作者：
T. Sarantou;D. Chi;D. Garrison;A. Conrad;P. Schmid;D. Morton;D. Hoon
影响因子：
11.2
作者：
D. Millikin;E. Meese;B. Vogelstein;C. Witkowski;Jeffrey M. Trent
通讯作者：
D. Millikin;E. Meese;B. Vogelstein;C. Witkowski;Jeffrey M. Trent
DOI：
10.1073/pnas.86.12.4614
发表时间：
1989-06-01
影响因子：
11.1
作者：
DRACOPOLI, NC;HARNETT, P;KEFFORD, RF
通讯作者：
KEFFORD, RF
影响因子：
158.5
作者：
JEN, J;KIM, HG;HAMILTON, SR
通讯作者：
HAMILTON, SR
缺失图谱揭示了结直肠癌中 8 号染色体等位基因的两个区域丢失。
DOI：
10.1002/gcc.2870100102
发表时间：
1994
期刊：
Genes, chromosomes & cancer
影响因子：
--
作者：
Yaremko,ML;Wasylyshyn,ML;Paulus,KL;Michelassi,F;Westbrook,CA
通讯作者：
Westbrook,CA