Mucosal and systemic immune responses in BALB/c mice to Bacteroides gingivalis fimbriae administered orally
Mucosal and systemic immune responses in BALB/c mice to Bacteroides gingivalis fimbriae administered orally
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BALB/c 小鼠对口服牙龈菌毛的粘膜和全身免疫反应
作者：
T. Ogawa;H. Shimauchi;S. Hamada
从新鲜培养的牙龈类杆菌381全细胞中分离纯化出一种相对分子质量为41,000的菌毛蛋白。唾液和血清对菌毛的抗体反应，该菌毛是在存在壁淀粉肽的酰基衍生物，即N2-[(N-acetylmuramyl)-L-alanyl-D-isoglutaminyl]-N6-stearoyl-L-lysine[MDP-Lys(L18)]或beta-N-acetyl-glucosaminyl-(1----4)-N-acetylmuramyl-L-alanyl-D-isoglu inyl-(L)-硬脂酰基-(D)-Meso-2，6-二氨基-(D)-D-丙氨酸(GM-53)的情况下口服的，或在无佐剂的情况下，分别在BALB/c小鼠第0天和第1天经胃插管，在第27天和第28天作为加强免疫进行检测。胃管插管菌毛和佐剂显著增加唾液中抗菌毛免疫球蛋白A(IgA)的产生。皮下注射菌毛和佐剂也提高了唾液中抗菌毛IgA水平和免疫球蛋白G水平。两种免疫方案均能提高血清中抗菌毛抗体的水平，且菌毛特异性抗体以免疫球蛋白G为主，其次为免疫球蛋白A和免疫球蛋白M。皮下注射比胃管注射能更有效地提高小鼠血清抗体的产生。血清中抗菌毛抗体亚类以IgG1为主，其次为IgG2a、IgG2b和IgG3。这些结果表明，牙周杆菌菌毛抗原与GM-53或MDP-Lys(L18)联合使用后，唾液中的IgA抗体反应和血清中以刺激为主的Ig G抗体反应分别显著增加。这两种抗体都被发现对用于免疫的菌毛具有特异性。
A 41,000-molecular-weight fimbrial protein was isolated from freshly cultivated whole cells of Bacteroides gingivalis 381 and purified chromatographically. Salivary and serum antibody responses to the fimbriae, which had been orally administered in the presence of an acyl derivative of muramylpeptides, i.e., either N2-[(N-acetylmuramyl)-L-alanyl-D-isoglutaminyl]-N6-stearoyl-L-lysine [MDP-Lys(L18)] or sodium beta-N-acetyl-glucosaminyl-(1----4)-N-acetylmuramyl-L-alanyl-D-isoglu tam inyl- (L)-stearoyl-(D)-meso-2,6-diaminopimelic acid-(D)-amine-D-alanine (GM-53), or in the absence of adjuvant, were examined in BALB/c mice when administered by gastric intubation on days 0 and 1 as primary immunizations and on days 27 and 28 as booster immunizations. Gastric intubation of the fimbriae with an adjuvant significantly enhanced the production of anti-fimbria immunoglobulin A (IgA) in saliva. Subcutaneous injection of fimbriae along with an adjuvant also raised anti-fimbria IgA levels, as well as IgG levels, in saliva. Both immunization procedures enhanced the levels of anti-fimbria IgG, IgA, and IgM in serum, and the major class of fimbria-specific antibody was IgG, followed by IgA and IgM. However, subcutaneous injection was more effective than gastric intubation to enhance the production of serum antibody in mice. The subclasses of IgG antibody specific for fimbriae in serum were mainly IgG1, followed by IgG2a, IgG2b, and IgG3. These results demonstrated that the combined use of B. gingivalis fimbrial antigen and either GM-53 or MDP-Lys(L18) resulted in a sharply increased IgA antibody response in saliva and a predominantly stimulated IgG antibody response in serum, respectively. Both antibodies were found to be specific for the fimbriae used for immunization.
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影响因子：
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作者：
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DOI：
--
发表时间：
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期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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DOI：
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发表时间：
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影响因子：
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DOI：
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影响因子：
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作者：
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