Early acquisition and high nasopharyngeal co-colonisation by Streptococcus pneumoniae and three respiratory pathogens amongst Gambian new-borns and infants.
Early acquisition and high nasopharyngeal co-colonisation by Streptococcus pneumoniae and three respiratory pathogens amongst Gambian new-borns and infants.
复制标题
DOI：
10.1186/1471-2334-11-175
复制
发表时间：
2011-06-20
影响因子：
3.7
通讯作者：
Antonio M
中科院分区：
文献类型：
--
作者：
Kwambana BA;Barer MR;Bottomley C;Adegbola RA;Antonio M
虽然b型流感嗜血杆菌(Hib)、金黄色葡萄球菌和卡他莫拉氏菌是儿童侵袭性和粘膜细菌性疾病的重要原因，但在西非尚未确定婴儿时期与肺炎链球菌共同携带。从30名冈比亚新生儿采集498份鼻咽拭子，每隔2周(0~6个月)和两个月(2个月至12个月)用物种特异性聚合酶链式反应(PCR)检测每种微生物。所有婴儿在婴儿期的几个时间点都携带肺炎链球菌、流感嗜血杆菌和卡他支原体。肺炎链球菌与至少一种其他病原体在婴儿鼻咽部共定植的次数为十分之九。新生儿和新生儿有较早的定植，肺炎链球菌、流感嗜血杆菌、卡他分支杆菌和金黄色葡萄球菌首次检出的平均时间分别为5、7、3和14周。肺炎链球菌、流感嗜血杆菌和卡他支原体在新生儿中的患病率升高，分别在出生后13、15和23周达到80%以上。相比之下，金黄色葡萄球菌在新生儿中的患病率从50%下降到9周大新生儿的20%。肺炎链球菌与流感嗜血杆菌(OR 5.03；95%CI 3.02，8.39；p<；0.01)和卡他支原体(OR 2.20；95%CI 1.29；p<；0.01)呈显著正相关，与金黄色葡萄球菌呈负相关(OR 0.53；95%CI 0.30，0.94；p=0.03)。这项研究表明，冈比亚新生儿和婴儿鼻咽粘膜中三种重要的呼吸道病原体与肺炎链球菌高度共携带。这对呼吸道多菌感染的病原学、生物被膜的形成和疫苗策略具有重要的潜在意义。
Although Haemophilus influenzae type b (Hib), Staphylococcus aureus and Moraxella catarrhalis are important causes of invasive and mucosal bacterial disease among children, co-carriage with Streptococcus pneumoniae during infancy has not been determined in West Africa. Species specific PCR was applied to detect each microbe using purified genomic DNA from 498 nasopharyngeal (NP) swabs collected from 30 Gambian neonates every two weeks from 0 to 6 months and bi-monthly up to 12 months. All infants carried S. pneumoniae, H. influenzae and M. catarrhalis at several time points during infancy. S.pneumoniae co-colonized the infant nasopharynx with at least one other pathogen nine out of ten times. There was early colonization of the newborns and neonates, the average times to first detection were 5, 7, 3 and 14 weeks for S. pneumoniae, H. influenzae, M. catarrhalis and S. aureus respectively. The prevalence of S. pneumoniae, H. influenzae and M. catarrhalis increased among the neonates and exceeded 80% by 13, 15 and 23 weeks respectively. In contrast, the prevalence of S. aureus decreased from 50% among the newborns to 20% amongst nine-week old neonates. S. pneumoniae appeared to have a strong positive association with H. influenzae (OR 5.03; 95% CI 3.02, 8.39; p < 0.01) and M. catarrhalis (OR 2.20; 95% CI 1.29; p < 0.01) but it was negatively associated with S. aureus (OR 0.53; 95% CI 0.30, 0.94; p = 0.03). This study shows early acquisition and high co-carriage of three important respiratory pathogens with S. pneumoniae in the nasopharyngeal mucosa among Gambian neonates and infants. This has important potential implications for the aetiology of respiratory polymicrobial infections, biofilm formation and vaccine strategies.
登录
查看更多内容
影响因子：
9.4
作者：
Maaroufi, Younes;De Bruyne, Jean-Marc;Crokaert, Francoise
通讯作者：
Crokaert, Francoise
影响因子：
5.5
作者：
Howie, Stephen R. C.;Antonio, Martin;Adegbola, Richard A.
通讯作者：
Adegbola, Richard A.
影响因子：
6.4
作者：
GRAY, BM;CONVERSE, GM;DILLON, HC
通讯作者：
DILLON, HC
影响因子：
6.4
作者：
Adegbola, RA;Mulholland, EK;Greenwood, BM
通讯作者：
Greenwood, BM
影响因子：
--
作者：
Kwambana BA;Mohammed NI;Jeffries D;Barer M;Adegbola RA;Antonio M
通讯作者：
Antonio M