Novel gene loci associated with susceptibility or cryptic quantitative resistance to Pyrenopeziza brassicae in Brassica napus.
Novel gene loci associated with susceptibility or cryptic quantitative resistance to Pyrenopeziza brassicae in Brassica napus.
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DOI：
10.1007/s00122-023-04243-y
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发表时间：
2023-03-23
影响因子：
5.4
通讯作者：
Stotz, Henrik U.
中科院分区：
文献类型：
--
作者：
Fell, Heather;Ali, Ajisa Muthayil;Wells, Rachel;Mitrousia, Georgia K.;Woolfenden, Hugh;Schoonbeek, Henk-Jan;Fitt, Bruce D. L.;Ridout, Christopher J.;Stotz, Henrik U.
利用数量抗病性（QDR）基因控制甘蓝型油菜轻斑病的发生，筛选出4个处于连锁不平衡的QDR位点和8个基因表达标记。与抗性（R）基因介导的抗性相比，数量抗病性（QDR）可以提供对作物中病原体的持久控制，抗性（R）基因介导的抗性可以由于病原体进化而被破坏。因此，QDR是作物改良的理想性状，但对致病基因知之甚少，因此难以纳入育种计划。甘蓝型油菜轻斑病是由油菜核盘菌（Pyrenopeziza brassicae）引起的油菜重要病害。为了鉴定新的QDR基因位点，我们在195个B系上使用了具有芸苔疫霉的高通量筛选病理系统。napus与关联转录组学平台相结合。结果表明，所有对甘蓝黑腐病菌的抗性都与QDR基因有关，而不是R基因介导的。我们使用全基因组关联分析与改进的B。油菜群体结构揭示四个基因位点显着（P = 0.0001）与QDR的区域显示连锁不平衡。在染色体A09上，增强的抗性与细胞色素P450基因的杂合性相关，该基因与先前描述的种子硫代葡萄糖苷含量的基因座共定位。此外，8个显著的基因表达标记与QDR对芸苔疫霉的假发现率为0.001。对于其中的7个，表达与抗性呈正相关，而对于一个，HXXXD型酰基转移酶，负相关表明潜在的易感基因。这项研究确定了新的QDR基因座的敏感性和抗性，包括新的隐性QDR基因与杂合性，这将为未来的作物改良。在线版本包含补充材料，可通过10.1007/s 00122 -023-04243-y获得。
Quantitative disease resistance (QDR) controls the association of the light leaf spot pathogen with Brassica napus; four QDR loci that were in linkage disequilibrium and eight gene expression markers were identified. Quantitative disease resistance (QDR) can provide durable control of pathogens in crops in contrast to resistance (R) gene-mediated resistance which can break down due to pathogen evolution. QDR is therefore a desirable trait in crop improvement, but little is known about the causative genes, and so it is difficult to incorporate into breeding programmes. Light leaf spot, caused by Pyrenopeziza brassicae, is an important disease of oilseed rape (canola, Brassica napus). To identify new QDR gene loci, we used a high-throughput screening pathosystem with P. brassicae on 195 lines of B. napus combined with an association transcriptomics platform. We show that all resistance against P. brassicae was associated with QDR and not R gene-mediated. We used genome-wide association analysis with an improved B. napus population structure to reveal four gene loci significantly (P = 0.0001) associated with QDR in regions showing linkage disequilibrium. On chromosome A09, enhanced resistance was associated with heterozygosity for a cytochrome P450 gene co-localising with a previously described locus for seed glucosinolate content. In addition, eight significant gene expression markers with a false discovery rate of 0.001 were associated with QDR against P. brassicae. For seven of these, expression was positively correlated with resistance, whereas for one, a HXXXD-type acyl-transferase, negative correlation indicated a potential susceptibility gene. The study identifies novel QDR loci for susceptibility and resistance, including novel cryptic QDR genes associated with heterozygosity, that will inform future crop improvement. The online version contains supplementary material available at 10.1007/s00122-023-04243-y.
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DOI：
10.1111/nph.17292
发表时间：
2021-08
期刊：
The New phytologist
影响因子：
--
作者：
Jiquel A;Gervais J;Geistodt-Kiener A;Delourme R;Gay EJ;Ollivier B;Fudal I;Faure S;Balesdent MH;Rouxel T
通讯作者：
Rouxel T
影响因子：
5.3
作者：
Wells R;Trick M;Fraser F;Soumpourou E;Clissold L;Morgan C;Pauquet J;Bancroft I
通讯作者：
Bancroft I
含核闸门的死盒RNA解旋酶在Phandcomitrium patens中控制着配子型和孢子型发育。
影响因子：
5.1
作者：
Perroud PF;Demko V;Ako AE;Khanal R;Bokor B;Pavlovič A;Jásik J;Johansen W
通讯作者：
Johansen W
DOI：
10.1111/tpj.13767
发表时间：
2018-01
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Havlickova L;He Z;Wang L;Langer S;Harper AL;Kaur H;Broadley MR;Gegas V;Bancroft I
通讯作者：
Bancroft I
影响因子：
16.6
作者：
An, Hong;Qi, Xinshuai;Pires, J. Chris
通讯作者：
Pires, J. Chris