Stimulated peripheral blood mononuclear cells from chlamydia-infected women release predominantly Th1-polarizing cytokines.
Stimulated peripheral blood mononuclear cells from chlamydia-infected women release predominantly Th1-polarizing cytokines.
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DOI：
10.1016/j.cyto.2018.06.017
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发表时间：
2019-01
期刊：
影响因子：
3.8
通讯作者：
Geisler WM
中科院分区：
文献类型：
--
作者：
Jordan SJ;Bakshi RK;Brown LT;Chi X;Geisler WM
沙眼衣原体感染(衣原体)是最常见的性传播细菌感染，在妇女中造成严重的生殖疾病。尽管衣原体的动物模型表明T辅助1型(Th1)反应对于衣原体清除和保护性免疫是重要的，但对女性对衣原体的免疫如何发展知之甚少，而T辅助2型(Th2)应答与持续感染有关。在衣原体感染的女性中，主要的免疫反应是Th1还是Th2极化仍然存在争议。为了确定衣原体感染妇女外周血单个核细胞(PBMC)激发的细胞因子谱，我们用沙眼衣原体基本体和重组沙眼衣原体Pgp3刺激PBMC，并检测跨越Th1和Th2极化反应的部分细胞因子的培养上清水平。我们发现，衣原体感染妇女刺激的PBMC分泌细胞因子，表明强烈的Th1极化反应，特别是干扰素-伽马，而Th2极化细胞因子的表达水平明显较低。在衣原体感染的妇女中，沙眼衣原体抗原刺激外周血单核细胞时，主要的细胞因子反应是Th1极化，其中干扰素-γ是主要的细胞因子。
Chlamydia trachomatis infection (chlamydia) is the most prevalent sexually transmitted bacterial infection and causes significant reproductive morbidity in women. Little is known about how immunity to chlamydia develops in women, though animal models of chlamydia indicate that T-helper type 1 (Th1) responses are important for chlamydia clearance and protective immunity, whereas T-helper type 2 (Th2) responses are associated with persisting infection. In chlamydia-infected women, whether the predominant immune response is Th1- or Th2-polarizing remains controversial. To determine the cytokine profiles elicited by peripheral blood mononuclear cells (PBMCs) from chlamydia-infected women, we stimulated PBMCs with C. trachomatis elementary bodies and recombinant C. trachomatis Pgp3 and measured supernatant levels of select cytokines spanning Th1- and Th2-polarizing responses. We found that stimulated PBMCs from chlamydia-infected women secreted cytokines that indicate strong Th1-polarizing responses, especially interferon-gamma, whereas Th2-polarizing cytokines were expressed at significantly lower levels. In chlamydia-infected women, the predominant cytokine responses elicited on stimulation of PBMCs with C. trachomatis antigens were Th1-polarizing, with interferon-gamma as the predominant cytokine.
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影响因子：
4.6
作者：
Hook, CE;Telyatnikova, N;Gaston, JSH
通讯作者：
Gaston, JSH
影响因子：
2.7
作者：
Lue, He-Zuo;Zhu, An-You;Li, Bai-Qing
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影响因子：
3.3
作者：
Mavoungou, E;Poaty-Mavoungou, V;Lansoud-Soukate, J
通讯作者：
Lansoud-Soukate, J
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3.4
作者：
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通讯作者：
Darville, Toni
影响因子：
3.1
作者：
Ito, JI;Lyons, JM
通讯作者：
Lyons, JM