Peripheral ghrelin stimulates feeding behavior and positive energy balance in a sciurid hibernator.
Peripheral ghrelin stimulates feeding behavior and positive energy balance in a sciurid hibernator.
复制标题
外围生长素蛋白刺激Sciurid Hibernator中的喂养行为和正能量平衡。
DOI：
10.1016/j.yhbeh.2011.01.013
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发表时间：
2011-04
影响因子：
3.5
通讯作者：
Florant, Gregory L.
中科院分区：
文献类型：
--
作者：
Healy, Jessica E.;Bateman, Jenna L.;Ostrom, Cara E.;Florant, Gregory L.
冬眠动物表现出强烈的体重增加和减少的年度循环，主要由食物摄入介导，但控制这些动物食物摄入的途径尚未完全阐明。胃饥饿素是一种促氧激素，可以增加所有哺乳动物的摄食量，但直到最近才在冬眠动物中进行研究。在其他哺乳动物中，胃饥饿素通过磷酸化和激活amp激活的蛋白激酶（AMPK）来刺激摄食。AMPK的激活使乙酰辅酶a羧化酶（ACC）磷酸化和失活，这是脂肪酸合成的一个重要步骤。为了确定外源性胃饥饿素对冬眠动物食物摄取量和代谢因子（即非酯化脂肪酸（NEFAs）、下丘脑AMPK和ACC）的影响，我们在四季中将胃饥饿素外周注射到地松鼠体内。在注射后的2-6小时内，记录了松鼠的食物摄入量和体重的变化，并对松鼠实施安乐死。取脑和血，Western blots检测下丘脑AMPK和ACC磷酸化的变化。采用比色法测定血清NEFAs浓度的变化。我们发现，与注射盐水的对照组相比，注射胃饥饿素的小鼠食物摄入量、体重和运动活动显著增加，即使是在冬季失语期注射的小鼠也是如此。注射ghrelin与AMPK磷酸化升高相关，但对冬季ACC无影响。与生理盐水对照组相比，注射生长素的动物血清NEFAs水平也有所提高。这项研究首次展示了注射胃饥饿素对冬眠动物的影响。
Hibernators exhibit a robust circannual cycle of body mass gain and loss primarily mediated by food intake, but the pathways controlling food intake in these animals have not been fully elucidated. Ghrelin is an orexigenic hormone that increases feeding in all mammals studied so far, but has not until recently been studied in hibernators. In other mammals, ghrelin stimulates feeding through phosphorylation and activation of AMP-activated protein kinase (AMPK). Activation of AMPK phosphorylates and deactivates acetyl Co-A carboxylase (ACC), a committed step in fatty acid synthesis. In order to determine the effects of exogenous ghrelin on food intake and metabolic factors (i.e. non-esterified fatty acids (NEFAs), and hypothalamic AMPK and ACC) in hibernators, ghrelin was peripherally injected into ground squirrels in all four seasons. Changes in food intake and body mass were recorded over a 2–6 hour period post injections, and squirrels were euthanized. Brains and blood were removed, and Western blots were performed to determine changes in phosphorylation of hypothalamic AMPK and ACC. A colorimetric assay was used to determine changes in concentration of serum NEFAs. We found that food intake, body mass, and locomotor activity significantly increased with ghrelin injections versus saline-injected controls, even in animals injected during their aphagic winter season. Injected ghrelin was correlated with increased phosphorylation of AMPK, but didn’t have an effect on ACC in winter. Ghrelin-injected animals also had increased levels of serum NEFAs compared with saline controls. This study is the first to show an effect of injected ghrelin on a hibernator.
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影响因子：
16.2
作者：
Cowley, MA;Smith, RG;Horvath, TL
通讯作者：
Horvath, TL
DOI：
10.1016/s0006-291x(02)02740-7
发表时间：
2002-12-20
影响因子：
3.1
作者：
Carlini, VP;Monzón, ME;de Barioglio, SR
通讯作者：
de Barioglio, SR
DOI：
10.1016/j.bbrc.2007.11.166
发表时间：
2008-02-08
影响因子：
3.1
作者：
Kohno, Daisuke;Sone, Hideyuki;Yada, Toshihiko
通讯作者：
Yada, Toshihiko
影响因子：
3.5
作者：
Jaszberenyi, M.;Bujdoso, E.;Telegdy, G.
通讯作者：
Telegdy, G.
影响因子：
3.5
作者：
Bradley, Sean P.;Pattullo, Lucia M.;Patel, Priyesh N.;Prendergast, Brian J.
通讯作者：
Prendergast, Brian J.