Repurposing CRISPR System for Transcriptional Activation.
Repurposing CRISPR System for Transcriptional Activation.
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DOI：
10.1007/978-981-10-4310-9_10
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发表时间：
2017
影响因子：
--
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中科院分区：
文献类型：
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近年来，CRISPR/Cas9系统已成为基因组编辑中最受欢迎的系统。当Cas9蛋白的核酸酶结构域突变为失活形式（dCas 9）时，CRISPR/dCas 9仍然保留结合靶向DNA序列的能力，但失去了核酸内切酶切割活性。利用这种工程化核酸酶失活Cas9的特性，CRISPR/dCas系统已被重新用于多功能RNA引导的DNA靶向平台，如基因组成像，基因调控和表观遗传修饰。具体地，dCas 9与激活结构域的融合允许在不同生物体中在全基因组范围内特异性和有效的转录激活。本章的目的是回顾最近发表的关于基于CRISPR/dCas 9的转录激活系统的重要文献。与用于增强特定感兴趣基因表达的常规方法相比，基于CRISPR/Cas9的系统已成为基因组调控的有前途的技术，其允许内源基因激活的特异性、方便性、鲁棒性和可扩展性。
In recent years, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has become the most popular one for genome editing. When the nuclease domains of Cas9 protein are mutated into deactivated form (dCas9), CRISPR/dCas9 still retains the ability to bind the targeted DNA sequence, but loses the endonuclease cleavage activity. Taking advantage of the characteristics of this engineered nuclease inactive Cas9, the CRISPR/dCas system has been repurposed into versatile RNA-guided, DNA-targeting platforms, such as genome imaging, gene regulation, and epigenetic modification. Specifically, fusion of dCas9 with activation domains allows specific and efficient transcriptional activation on a genome-wide scale among diverse organisms. The purpose of this chapter is to review most important the recently published literature on CRISPR/dCas9-based transcriptional activation systems. Compared with the conventional approaches for enhancement of the expression of specific genes of interest, CRISPR/Cas9-based system has emerged as a promising technology for genome regulation, allowing specificity, convenience, robustness, and scalability for endogenous gene activation.
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影响因子：
64.8
作者：
Konermann S;Brigham MD;Trevino AE;Joung J;Abudayyeh OO;Barcena C;Hsu PD;Habib N;Gootenberg JS;Nishimasu H;Nureki O;Zhang F
通讯作者：
Zhang F
影响因子：
48
作者：
Chavez A;Tuttle M;Pruitt BW;Ewen-Campen B;Chari R;Ter-Ovanesyan D;Haque SJ;Cecchi RJ;Kowal EJK;Buchthal J;Housden BE;Perrimon N;Collins JJ;Church G
通讯作者：
Church G
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
DOI：
10.1126/science.1232033
发表时间：
2013-02-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Mali P;Yang L;Esvelt KM;Aach J;Guell M;DiCarlo JE;Norville JE;Church GM
通讯作者：
Church GM
CRISPR/CAS9用于人类基因组工程和疾病研究。
DOI：
10.1146/annurev-genom-083115-022258
发表时间：
2016-08-31
影响因子：
8.7
作者：
通讯作者：
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