Accumulation of the RNA polymerase subunit RpoB depends on RNA editing by OsPPR16 and affects chloroplast development during early leaf development in rice.
Accumulation of the RNA polymerase subunit RpoB depends on RNA editing by OsPPR16 and affects chloroplast development during early leaf development in rice.
复制标题
RNA 聚合酶亚基 RpoB 的积累取决于 OsPPR16 的 RNA 编辑，并影响水稻早期叶片发育过程中的叶绿体发育。
DOI：
10.1111/nph.16769
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发表时间：
2020-11
期刊：
影响因子：
--
通讯作者：
Zhou F
中科院分区：
文献类型：
--
作者：
Huang W;Zhang Y;Shen L;Fang Q;Liu Q;Gong C;Zhang C;Zhou Y;Mao C;Zhu Y;Zhang J;Chen H;Zhang Y;Lin Y;Bock R;Zhou F
质体编码的基因由核编码的RNA聚合酶（NEP）和质体编码的RNA聚合酶（PEP）协同转录。由此产生的初级转录物经常通过在特定位点的胞苷到尿苷的转化进行RNA编辑。许多编辑事件的生理作用在很大程度上是未知的。在这里，我们在水稻中使用了CRISPR/Cas9技术来敲除五肽重复序列（PPR）蛋白的PLS-DYW亚家族成员。我们发现OsPPR 16负责叶绿体rpoB信使RNA（mRNA）中545位的单一编辑事件，导致PEP β亚基中的氨基酸从丝氨酸变为亮氨酸。与拟南芥中推定的直向同源物的功能丧失突变（据报道其没有可见的表型）形成鲜明对比，OsPPR 16的敲除导致RpoB积累受损，PEP依赖性基因表达减少，以及植物发育早期的苍白表型。因此，通过编辑rpoB mRNA，OsPPR 16是忠实的质体转录所必需的，而质体转录又是Chl合成和高效叶绿体发育所必需的。我们的研究结果为在质体基因表达的转录和转录后水平上运作的微调调控机制的相互联系提供了新的见解。
Plastid‐encoded genes are coordinately transcribed by the nucleus‐encoded RNA polymerase (NEP) and the plastid‐encoded RNA polymerase (PEP). Resulting primary transcripts are frequently subject to RNA editing by cytidine‐to‐uridine conversions at specific sites. The physiological role of many editing events is largely unknown. Here, we have used the CRISPR/Cas9 technique in rice to knock out a member of the PLS‐DYW subfamily of pentatricopeptide repeat (PPR) proteins. We found that OsPPR16 is responsible for a single editing event at position 545 in the chloroplast rpoB messenger RNA (mRNA), resulting in an amino acid change from serine to leucine in the β‐subunit of the PEP. In striking contrast to loss‐of‐function mutations of the putative orthologue in Arabidopsis, which were reported to have no visible phenotype, knockout of OsPPR16 leads to impaired accumulation of RpoB, reduced expression of PEP‐dependent genes, and a pale phenotype during early plant development. Thus, by editing the rpoB mRNA, OsPPR16 is required for faithful plastid transcription, which in turn is required for Chl synthesis and efficient chloroplast development. Our results provide new insights into the interconnection of the finely tuned regulatory mechanisms that operate at the transcriptional and post‐transcriptional levels of plastid gene expression.
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