New Insights into Avian Infectious Bronchitis Virus in Colombia from Whole-Genome Analysis.
New Insights into Avian Infectious Bronchitis Virus in Colombia from Whole-Genome Analysis.
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DOI：
10.3390/v14112562
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发表时间：
2022-11-19
期刊：
影响因子：
--
通讯作者：
Gomez AP
中科院分区：
文献类型：
--
作者：
Ramirez-Nieto G;Mir D;Almansa-Villa D;Cordoba-Argotti G;Beltran-Leon M;Rodriguez-Osorio N;Garai J;Zabaleta J;Gomez AP
传染性支气管炎（IB）是由高度变异的γ冠状病毒引起的呼吸道疾病，对全球家禽健康产生负面影响。GI-11和GI-16谱系已根据传染性支气管炎病毒（IBV）部分S1序列在南美洲鉴定。然而，全基因组序列信息是有限的。在这项研究中，我们报告，第一次，全基因组序列的IBV从哥伦比亚。选择2012年和2013年期间从具有与IB相容的呼吸道疾病的农场获得的7个IBV分离株，并通过NGS获得全基因组序列。根据S1序列系统发育分析，6个菌株属于GI-1谱系，1个菌株属于GVI-1谱系。当分析全基因组时，5个分离株与疫苗株Ma5 2016相关，2个显示嵌合基因组。从完整的S1序列分析的结果提供了进一步的支持的假设，GVI-1，被认为是一个地理局限的血统在亚洲，可能起源于哥伦比亚。本研究报告的完整基因组信息使人们能够更深入地了解变异株的系统发育进化以及在全球范围内传播的毒株之间的重组事件，有助于了解拉丁美洲和世界的冠状病毒。
Infectious Bronchitis (IB) is a respiratory disease caused by a highly variable Gammacoronavirus, which generates a negative impact on poultry health worldwide. GI-11 and GI-16 lineages have been identified in South America based on Infectious Bronchitis virus (IBV) partial S1 sequences. However, full genome sequence information is limited. In this study we report, for the first time, the whole-genome sequence of IBV from Colombia. Seven IBV isolates obtained during 2012 and 2013 from farms with respiratory disease compatible with IB were selected and the complete genome sequence was obtained by NGS. According to S1 sequence phylogenetic analysis, six isolates belong to lineage GI-1 and one to lineage GVI-1. When whole genome was analyzed, five isolates were related to the vaccine strain Ma5 2016 and two showed mosaic genomes. Results from complete S1 sequence analysis provides further support for the hypothesis that GVI-1, considered a geographically confined lineage in Asia, could have originated in Colombia. Complete genome information reported in this research allow a deeper understanding of the phylogenetic evolution of variants and the recombination events between strains that are circulating worldwide, contributing to the knowledge of coronavirus in Latin America and the world.
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影响因子：
14.9
作者：
Letunic I;Bork P
通讯作者：
Bork P
影响因子：
3.7
作者：
Jackwood MW;Boynton TO;Hilt DA;McKinley ET;Kissinger JC;Paterson AH;Robertson J;Lemke C;McCall AW;Williams SM;Jackwood JW;Byrd LA
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Byrd LA
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10.7
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Minh BQ
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1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM