A 3D human adipose tissue model within a microfluidic device.
A 3D human adipose tissue model within a microfluidic device.
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DOI：
10.1039/d0lc00981d
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发表时间：
2021-01-21
期刊：
影响因子：
6.1
通讯作者：
Brey EM
中科院分区：
文献类型：
--
作者：
Yang F;Carmona A;Stojkova K;Garcia Huitron EI;Goddi A;Bhushan A;Cohen RN;Brey EM
一种精确的人体脂肪组织体外模型有助于研究脂肪细胞的功能，并为筛选新的治疗化合物提供更好的工具。二维表面的细胞培养模型无法模拟体内脂肪的三维环境，而三维培养模型往往无法支持长期细胞培养，部分原因是物质传递不足。微流体系统已被用于脂肪组织模型的研究。然而，目前的系统主要集中在二维培养的脂肪细胞上。在这项工作中，在微流体系统内构建了一种三维人体脂肪微组织。人脂肪来源的干细胞（ADSCs）被用作产生分化脂肪细胞的细胞来源。在微流体系统内分化的ADSCs形成了一个富含脂质的致密团块，并表达脂肪组织的基因标记。工程化的脂肪组织显示，随着剪切应力的增加，脂联素分泌减少，游离脂肪酸分泌增加。随着剪切应力的增加，脂肪生成标记物下调。总体而言，这种微流体系统能够在芯片上实现功能性三维人体脂肪微组织在间质流作用下的分化和发育。该系统有可能作为一个平台，用于脂肪组织相关疾病的体外药物测试。
这项工作展示了一种用于在可控流动条件下构建和分析三维人体脂肪组织的微流体系统。
An accurate in vitro model of human adipose tissue could assist in the study of adipocyte function and allow for better tools for screening new therapeutic compounds. Cell culture models on two-dimensional surfaces fall short of mimicking the three-dimensional in vivo adipose environment, while three-dimensional culture models are often unable to support long-term cell culture due, in part, to insufficient mass transport. Microfluidic systems have been explored for adipose tissue models. However, current systems have primarily focused on 2D cultured adipocytes. In this work, a 3D human adipose microtissue was engineered within a microfluidic system. Human adipose-derived stem cells (ADSCs) were used as the cell source for generating differentiated adipocytes. The ADSCs differentiated within the microfluidic system formed a dense lipid-loaded mass with the expression of adipose tissue genetic markers. Engineered adipose tissue showed a decreased adiponectin secretion and increased free fatty acid secretion with increasing shear stress. Adipogenesis markers were downregulated with increasing shear stress. Overall, this microfluidic system enables the on-chip differentiation and development of a functional 3D human adipose microtissue supported by the interstitial flow. This system could potentially serve as a platform for in vitro drug testing for adipose tissue-related diseases. This work presents a microfluidic system for the engineering and analysis of 3D human adipose tissue under controlled flow.
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DOI：
10.1530/joe-13-0339
发表时间：
2014-02
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Cao H
通讯作者：
Cao H
DOI：
10.1039/c4an01725k
发表时间：
2015-02-21
期刊：
The Analyst
影响因子：
--
作者：
Godwin LA;Brooks JC;Hoepfner LD;Wanders D;Judd RL;Easley CJ
通讯作者：
Easley CJ
影响因子：
3
作者：
Gerlach, Joerg C.;Lin, Yen-Chih;Marra, Kacey G.
通讯作者：
Marra, Kacey G.
影响因子：
5.6
作者：
Jung UJ;Choi MS
通讯作者：
Choi MS
影响因子：
6.1
作者：
Hsu YH;Moya ML;Hughes CC;George SC;Lee AP
通讯作者：
Lee AP