Ultra-performance liquid chromatography-mass spectrometry-based metabolomics reveals Huangqiliuyi decoction attenuates abnormal metabolism as a novel therapeutic opportunity for type 2 diabetes.
Ultra-performance liquid chromatography-mass spectrometry-based metabolomics reveals Huangqiliuyi decoction attenuates abnormal metabolism as a novel therapeutic opportunity for type 2 diabetes.
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基于超高效液相色谱-质谱法的代谢组学揭示黄芪六益汤减轻异常代谢是2型糖尿病的新治疗机会
作者：
背景：2型糖尿病作为一种典型的慢性代谢性疾病，给社会造成了沉重的医疗负担。本研究应用代谢组学方法探讨黄芪六一汤治疗2型糖尿病的分子机制。采用超高效液相色谱-质谱联用技术结合模式识别方法筛选与HQLYD密切相关的代谢产物。运用生物标志物通路分析和生物网络分析等方法，探讨黄芪甘草汤的治疗作用及作用机制。在动物模型中鉴定了总共25种生物标志物，其中16种生物标志物与HQLYD治疗T2 D相关。它们减弱了代谢途径的异常，如苯丙氨酸、酪氨酸和色氨酸生物合成、苯丙氨酸代谢和柠檬酸循环。HQLYD还能显著升高血清FINS和肝、肾组织SOD、GSP-x水平，降低血清TC、TG、HDL、LDL、尿素、Scr、AST、ALT、FBG、IRS、MDA、CAT水平。结果表明，黄芪甘草汤治疗2型糖尿病的作用机制可能与氨基酸代谢、糖代谢和脂代谢有关。代谢组学研究表明黄芪六一汤改善代谢异常是治疗2型糖尿病的新途径。
Background: As a typical chronic metabolic disease, type 2 diabetes mellitus causes a heavy health-care burden to society. In this study, we applied the metabolomics strategy to explore the potential molecular mechanism of the Huangqiliuyi decoction (HQLYD) for type-2 diabetes (T2D). Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) combined with pattern recognition methods was utilized to select specific metabolites closely associated with HQLYD. Biomarker pathway analysis and biological network were utilized to uncover the therapeutic effect and action mechanism related to HQLYD. A total of twenty-five biomarkers were identified in the animal model, in which sixteen biomarkers are associated with HQLYD treatment for T2D. They attenuated the abnormalities of metabolic pathways such as phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, and the citrate cycle. HQLYD also significantly elevated the serum FINS and SOD, GSP-x level in the liver and kidney, and reduced the serum TC, TG, HDL, LDL, urea, Scr, AST, ALT, FBG, IRS, MDA, and CAT level. We found that the therapeutic mechanism of HQLYD against T2D affected amino acid metabolism, glucose metabolism and lipid metabolism. Metabolomics revealed that the Huangqiliuyi decoction attenuates abnormal metabolism as a novel therapeutic opportunity for type 2 diabetes.
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