The N-terminal region of the PA subunit of the RNA polymerase of influenza A/HongKong/156/97 (H5N1) influences promoter binding.
The N-terminal region of the PA subunit of the RNA polymerase of influenza A/HongKong/156/97 (H5N1) influences promoter binding.
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DOI：
10.1371/journal.pone.0005473
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Brownlee GG
中科院分区：
文献类型：
--
作者：
Kashiwagi T;Leung BW;Deng T;Chen H;Brownlee GG
流感病毒的RNA聚合酶是PB 1、PB 2和PA亚基的异源三聚体复合物，它们在病毒基因组的转录和复制中协作。先前的研究表明，A/WSN/33（H1N1）流感病毒PA亚基的N-末端区域参与启动子结合。在这里，我们扩展我们的研究流感RNA聚合酶的流感病毒株A/香港/156/97（H5 N1）和A/越南/1194/04（H5 N1）。最初于1997年和2004年从患者中分离的两种H5 N1毒株在体外显示出比两种经典的人类毒株A/WSN/33（H1N1）和A/NT/60/68（H3 N2）显著更高的聚合酶活性。这种增加的聚合酶活性与增强的启动子结合相关。PA亚基的N-末端区域是这种增强的启动子活性的主要决定因素。总的来说，我们认为，最近的两个H5 N1毒株的PA亚基的N-末端区域可以影响启动子结合，我们推测这可能是一个因素，在他们的毒力。
The RNA polymerase of influenza virus is a heterotrimeric complex of PB1, PB2 and PA subunits which cooperate in the transcription and replication of the viral genome. Previous research has shown that the N-terminal region of the PA subunit of influenza A/WSN/33 (H1N1) virus is involved in promoter binding. Here we extend our studies of the influenza RNA polymerase to that of influenza strains A/HongKong/156/97 (H5N1) and A/Vietnam/1194/04 (H5N1). Both H5N1 strains, originally isolated from patients in 1997 and 2004, showed significantly higher polymerase activity compared with two classical human strains, A/WSN/33 (H1N1) and A/NT/60/68 (H3N2) in vitro. This increased polymerase activity correlated with enhanced promoter binding. The N-terminal region of the PA subunit was the major determinant of this enhanced promoter activity. Overall we suggest that the N-terminal region of the PA subunit of two recent H5N1 strains can influence promoter binding and we speculate this may be a factor in their virulence.
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