Early pathogenesis of the foot-and-mouth disease virus O/JPN/2010 in experimentally infected pigs.
Early pathogenesis of the foot-and-mouth disease virus O/JPN/2010 in experimentally infected pigs.
复制标题
DOI：
10.1292/jvms.17-0683
复制
发表时间：
2018-04-27
期刊：
影响因子：
--
通讯作者：
Yamakawa M
中科院分区：
文献类型：
--
作者：
Yamada M;Fukai K;Morioka K;Nishi T;Yamazoe R;Kitano R;Shimada N;Yoshida K;Kanno T;Sakamoto K;Yamakawa M
我们研究了猪口蹄疫病毒（FMDV）O/JPN/2010感染早期多系统器官中病变的组织学分布以及与病毒和病毒RNA相关的病毒抗原。在猪中通过自然感染途径接种106组织培养感染剂量（TCID）50/ml的FMDV O/JPN/2010后3天（dpi），FMD猪中常见的特征性病变出现。然而，接种103 TCID 50/ml FMDV O/JPN/2010的6头猪在6 dpi内均未显示任何感染迹象。FMDV抗原的免疫组化检测和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）表明，FMDV主要感染棘细胞在棘层的舌头，冠状和球的脚跟，并导致这些感染的细胞进行细胞凋亡死亡。然而，没有证据表明FMDV O/JPN/2010感染基底层中的上皮/表皮基底细胞。病毒抗原阳性的舌上皮病变主要分布在舌背表面，而不分布在舌乳头、舌体和舌下表面。在3 dpi时，在所有3头接受检查的猪中均观察到非化脓性心肌炎和食管上皮病变（含FMDV抗原）。
We examined the histological distribution of the lesions and the viral antigen associated with the virus and virus RNA in multisystemic organs in the early stages of foot-and-mouth disease virus (FMDV) O/JPN/2010 infection in pigs. Characteristic lesions commonly observed in pigs with FMD arise following inoculation with 106 tissue culture infectious dose (TCID)50/ml of FMDV O/JPN/2010 in pigs at 3 days post inoculation (dpi) by a natural infectious route. However, none of the six pigs inoculated with 103 TCID50/ml of FMDV O/JPN/2010 showed any evidence of infection up to 6 dpi. Immunohistochemical detection for the FMDV antigen and terminal deoxynucleotidyl transferase-mediated dUTP-nick end labeling (TUNEL) showed that FMDV predominantly infected prickle cells in the stratum spinosum in the tongue, coronet and bulb of the heel, and caused these infected cells to undergo cell death by apoptosis. However, there was no evidence that FMDV O/JPN/2010 infected epithelial/epidermal basal cells in the basal layer. Epithelial lesions with viral antigen in the tongue were distributed in the dorsal surface but not in the papillae, corpus linguae or inferior surface of the tongue. Non-suppurative myocarditis and epithelial lesions in the esophagus with FMDV antigen were observed in all three pigs examined at 3 dpi.
登录
查看更多内容
影响因子：
2.7
作者：
Onozato, Hiroyuki;Fukai, Katsuhiko;Kanno, Toru
通讯作者：
Kanno, Toru
影响因子：
2.1
作者：
Stenfeldt, Carolina;Pacheco, Juan M.;Arzt, Jonathan
通讯作者：
Arzt, Jonathan
影响因子：
1.2
作者：
Sakamoto, K;Kanno, T;Murakami, Y
通讯作者：
Murakami, Y
影响因子：
3.8
作者：
Alexandersen, S;Oleksiewicz, MB;Donaldson, AI
通讯作者：
Donaldson, AI
DOI：
10.1002/ar.21534
发表时间：
2012-04-01
影响因子：
2
作者：
Liu, Zhixue;Yu, Qinghua;Yang, Qian
通讯作者：
Yang, Qian